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We narrowed to 286 results for: prep

Showing: 141 - 160 of 286 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... have all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions... sufficient quantity of DNA is produced from a miniprep and “low copy” if special growth conditions are... you a deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your...

  2. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...region. Thankfully, due to their prominence, the preparation of the fiber optics is probably the least difficult...effect is also an important consideration when preparing the implant itself, which brings me to the second...same considerations apply to the polishing and preparation of the patch cable (although you may be able ...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...conference, what worked? Use this information to prepare for the next meeting. Navigating talks Before the...publication, consider uploading your work to a preprint server (and include this information on the poster...later on. Update your LinkedIn and your resume and prepare business cards. You might not consider being a ...

  4. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...fragment can be used directly in your assemblies. Preparing reagents Keep it cool You’ve done all the learning...number of assemblies, you will also likely need to miniprep a lot of plasmids from your bacterial stocks beforehand...protocol shared by Dr. Rob Hurt for high-throughput minipreps in 96-well plates. Plate wisely You will likely...

  5. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...share- as opposed to sell- science. 4. You can prepare answers beforehand. Joanne answered many questions...incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of doing the AMA were interesting...interesting. I started watching the questions and preparing answers early in the day. Although I didn’t start...

  6. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or a maxiprep. In...

  7. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...fidelity. In this method, substrate pools were prepared consisting of one target (template) strand and... were represented. Sixteen separate pools were prepared, each with a different complement strand covering...applications such as routine cloning or DNA library preparation. However, when a method relies on accurate ligation...

  8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus...without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted epitope...temperature for 10 min. Deliver csRNP into cells: Prepare cells for transfection, add csRNP and ssODN to ...

  9. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...quest. Keep an eye out for Abi as they help us prepare for our upcoming antibody launch in late March!...

  10. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...gene expression! We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool...Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool...

  11. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...that too few of these trainees are sufficiently prepared during their 6-12+ (!) years of training to get... finding ways to provide new types of career preparedness training for their scientists. I have worked...

  13. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...conduct a two hour long interview. I anxiously prepared for the call. The length of this interview was...was to do for my time at Addgene. This training prepared me for the independent work on the QC team. I ...

  14. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ... Already we’ve seen articles about COVID-19 on preprint servers and in journals, and researchers have ...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports...

  15. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...for a successful application. It takes time to prepare an excellent resume…it is worth it. Read here to... to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and ...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  17. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...ahead of time so that lab members have time to prepare their thoughts. Then at the lab meeting, the team...everyone stands up, disappears from the video, and prepares a coffee, or simply moves away from the desk to...

  18. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...Whether you're working with cell cultures, slice preparations, or in vivo imaging, there's a version that ...cultures, zebrafish, and mouse brain, check out the preprint on bioRxiv. {% module_block module "widget_221e8fe4...
Showing: 141 - 160 of 286 results