We narrowed to 281 results for: prep
-
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....share- as opposed to sell- science. 4. You can prepare answers beforehand. Joanne answered many questions...incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of doing the AMA were interesting...interesting. I started watching the questions and preparing answers early in the day. Although I didn’t start...
-
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or a maxiprep. In... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....fidelity. In this method, substrate pools were prepared consisting of one target (template) strand and... were represented. Sixteen separate pools were prepared, each with a different complement strand covering...applications such as routine cloning or DNA library preparation. However, when a method relies on accurate ligation... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus...without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted epitope...temperature for 10 min. Deliver csRNP into cells: Prepare cells for transfection, add csRNP and ssODN to ... -
Our New Antibody Mascot is....
TypeBlog PostPublishedFeb. 24, 2022, 2:15 p.m....our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...quest. Keep an eye out for Abi as they help us prepare for our upcoming antibody launch in late March!... -
New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....gene expression! We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool...Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog PostPublishedSept. 2, 2014, 3:05 p.m....that too few of these trainees are sufficiently prepared during their 6-12+ (!) years of training to get... finding ways to provide new types of career preparedness training for their scientists. I have worked... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....particular phage researcher may only know how to prepare phage for one application but not another or they...Directory have the resources or know what it takes to prepare phages for clinical use. There are also different... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m.... Already we’ve seen articles about COVID-19 on preprint servers and in journals, and researchers have ...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....conduct a two hour long interview. I anxiously prepared for the call. The length of this interview was...was to do for my time at Addgene. This training prepared me for the independent work on the QC team. I ... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....for a successful application. It takes time to prepare an excellent resume…it is worth it. Read here to... to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m.... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....ahead of time so that lab members have time to prepare their thoughts. Then at the lab meeting, the team...everyone stands up, disappears from the video, and prepares a coffee, or simply moves away from the desk to... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....as exciting as it is challenging. How do you prepare for this shift and navigate the pitfalls? Here'...team success. This shift requires deliberate preparation and a willingness to embrace new responsibilities... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m....unwanted bacteria. How did graduate school help prepare you for the roles and skills necessary to be a ...constantly practicing. “The expenditure of effort in preparation and practice is directly related to success presenting... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....brought into the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing...of single clones obtained (on occasion we have prepared two 48-well plates of clones) which enables investigators... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....a three color panel or a 40 color panel! When preparing a spectral flow experiment and panel, there are...first run a new panel on a spectral cytometer, you prepare reference controls of both beads and cells for ... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....group dependencies Take, for instance, a recent preprint released by the Boehm and Beroukhim labs at the...ones made available through the work done in this preprint. Resources and references References Germline... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....package. There are three steps to chalk talk preparation: Define your research vision. Organize your ...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....space where you can address the issue head on. Prepare for this time as if it were a meeting. Gather the...change with them. If you can, set aside time to prepare for future challenges. This doesn’t have to be ...