...sequence! In bacteria, Cas13d-mediated cleavage promotes collateral cleavage of other RNAs. As with other...displays very high on-target efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx...

...science and engineering. “Fix the Institutions” promotes gender equality in careers through structural ...factors may include cell type, the disease being treated, and other hormonal, immunological, and environmental...

...binding proteins with Cas9 guided by different promoters. The challenge in building genetic circuits is...anywhere else, but for synthetic circuits, they are extremely orthogonal. It’s a different perspective and you...

...dopamine is an important neurotransmitter that promotes reward-motivated behavior, if the channelrhodopsins...as predict the channelrhodopsin’s photocurrent strength, wavelength sensitivity, and off-kinetics.   This...did not turn. Left-turning behavior in ChRger2-treated mice ceased when the light was turned off.  Together...

...gene expression and the selection of specific promoters may also help AAV vectors evade host immunity....potentially be treated by correcting the mutation or replacing the gene. A notable example is the treatment of Severe...conditions, opening up new avenues for therapeutics that treat disease at the molecular level. For instance, if...function. To date, gene therapy has been used to treat 10 infants with this disease. To do so, their T-...childhood onset of blindness and was thought to be untreatable until 2008. Following a single dose of subretinal...in vision over a period of 1 to 3 years – the treatment appears to be both safe and effective. Challenges...antibodies that neutralize the AAV vectors used to treat them. Scientists are working to overcome this issue...

...discussions. Get your voice out there. Use Twitter to promote your other science communication activities, e....engagement - Participate in science festivals and outreach If you are comfortable talking in public, volunteer...groups, charities and NGOs are often looking for outreach volunteers as well. 4) Blog - Hone your writing... a blog is not only a great form of scientific outreach, it also helps you to hone your writing skills...science communication. The National Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site ...

...incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated...experience of end-users, size of the transgene, and downstream applications should all be considered when choosing...factors such as resources available, cost and downstream applications. For example, while the PEG precipitation...be the best method when pure virus is required downstream. When assessing lentiviral titer, don't compare...

...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...others in Boston (some of them literally across the street from these institutions) are raising salaries. ...

...its paralog RC2H2 are RNA binding proteins that promote RNA decay and their upregulation is correlated ...little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared ...

...Program at Harvard Law School. He has continually promoted the cause of increasing data collection about ...Symposium Boston 2015 you can register here or live stream the event on October 22nd and 23rd here. Many ...

...orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...

...under the control of Murine Stem Cell retrovirus promoter for mammalian gene knockdown. Vector provided ...sequencing to perform multiplexed analysis of cells pre-treated with a compound of interest. Lentiviral vectors...

... had multiple integration events. In addition, promoter choice can also impact transgene expression. The...transduced with serial dilutions of a viral prep, treated with the antibiotic, and colony-forming units quantified...

...surprise since the PAM flexibility could in theory promote off-target binding. The activity of SpRY was validated...precise targeting (give or take a bp or two upstream or downstream) for most gene targeting experiments. Between...

...decreases. Hypermethylation of CpG islands in the promoter region of tumor suppressor genes can repress their...study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving...

...universities stressful or simply not feasible. Through the virtual systems created and promoted during COVID...fit for you without the financial and logistical stress that accompanies traveling for campus visits. This...have to worry about traveling during an already stressful time, and I was more relaxed during both sets ...their end. Though the quick changes in plans was stressful in and of itself, the flexibility allowed through...using the virtual world to your advantage can strengthen your application and help you decide if a program...

...complexed with a gRNA that directs them to the promoter of a gene of interest, they have been shown to...conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools...complex. When this complex is directed to a target upstream of the transcriptional start site (TSS) of a gene...competent to cut DNA. When directed to a site downstream of the transcriptional start site of a gene of...

... al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.... Test dTAG molecule toxicity in new cell lines Treat parental cells (or cells expressing a control --...

...express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library...Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-...

...gene expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...