...if you want the brightest signal, you may need to try several proteins in your system of interest. Finally...

...diagnostic restriction digest. For your digest: Try choosing unique enzymes. Enzymes that only cut once...

...any special tips or tricks for someone looking to try these optogenetic tools out for the first time? Boyden...

...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...be in and usually self-limiting effects kick in trying to resolve the inflammatory state by inducing immunosuppressive...

...with open data, materials, and code Only when you try to replicate the work of other researchers (or your...

...best astrocyte differentiation protocol! Ready to try it out for yourself? Check out the MORF Collection...

...absolutely everything was correct. Final touches We try to use figures and images to help illustrate our ... as well as consults with microscopy and flow cytometry experts for the panel recommendations. A few discussions...

...engage my students in real-world lab activities? I’m trying to inspire them and also keep up with all the new... the summer with dozens of teachers around the country. How you can get involved with BioBuilder Details...

...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress...researchers or students beginning to learn about biochemistry. The least this app will do is allow you to ...

...advance and make a note of those you would like to try and meet. Don’t worry about contacting someone much...

...software tools, what problems various tools are trying to solve, and finally how we might proceed in the...efficacy is still a concept that only a few tools are trying to solve. Advanced functions: These functions...different scoring algorithms and parameters. This tapestry of ideas is what makes choosing the right tool...

...expression levels, give these stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo...

... type tested. To conclude, we recommend that you try a couple of different fluorescent proteins when protein...

...with fluorescent proteins, the best advice is to try a couple of promising variants to check how they ...

...2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities....

...the post. And the blog writing tips are elements I try to include in each post I write for the Addgene blog...started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media as a scientist...) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social media as...

...next section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational... to other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in ...

...and ready to deliver. Ready to give biosensors a try? Peruse our available biosensors or build your own...biosensors span the gamut of applications within chemistry, biology, and physics, in this blog we will focus...

...others are dubious enough that they don’t give it a try. Optimization Why is GCE so complicated? Well, while... Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/...