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Showing: 801 - 810 of 866 results

  1. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time to check ...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory elements...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...

  3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...a method that uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a ...bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection. ...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Preger et al., 2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289...expression selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV ...

  5. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...Also, it remains challenging to screen for the genetically modified strains that contain your newly tagged...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... to deaminate cytidine and used dRanCas13b (catalytically inactive Cas13) to target the cytosine deaminase...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

  7. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...created by making some or all of the following modifications: 1) increased concentration of Taq polymerase...stretches of sequence could be used. However, a practical limitation is sequencing depth and cost. You need...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... the open conformation (Cofsky et al., 2022), indicating that there is more to learn about this process...conformational state of the HNH domain also allosterically controls RuvC activity through a hinge region...

  9. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... Western blot and share tips for this tricky application.    You can check out all of our protocols ...

  10. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...share quick tips to help you master fundamental technical lab skills, like pipetting! We’ll release a new...
Showing: 801 - 810 of 866 results