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... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...

...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

... of the entire plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing...

... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

...have good antibodies. The results look almost identical. Addgene: What advantages does your system have...

.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

...contracts behind closed doors. Fig. 1: The mythical land of negotiations.    You negotiate everyday...

...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

...-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine ...

...help you do your job better. #8 Thou shalt enthusiastically foster independence Your partner can make ...