...proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects....1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian O’Neill The Cohen...created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion protein...ticker tape images could recover transcriptional histories, accurate to within 40 minutes. Figure ...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

...control by Addgene before being distributed to customers. Details about our production protocols, titering...vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either performed by...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...The preparations are then aliquoted, frozen, and stored at -80 °C. Concentrated lentiviral preparations...then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All titering is performed on lentiviral... lentiviral preparations that have been stored at -80 °C and thawed. This ensures that any loss of titer...

...structures (at < 5 Å resolution) of the linker histone H1.8 in Xenopus nucleosomes and in complex with...has developed a new epigenetic editor, Coupled Histone tail for Autoinhibition Release of Methyltransferase...can be toxic, CHARM uses a cofactor fused to a histone tail to recruit and stimulate the endogenous methyltransferase...uses a fusion of the DNMT3L cofactor (D3L) with a histone tail (H3K4me0) to a DNA-binding protein (dCas9 ...team to develop self-silencing CHARM variants that stop their own expression after silencing their target... a familiar challenge for anyone who has done histology on brain tissue is identifying the cells you are...

.... We are the Customer Support Specialists here at Addgene. If you’ve called our customer service line ...'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...really getting at one bigger question: How does a customer place an Addgene order using a PO number? Why ...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,...

... Center for Regenerative Medicine of Boston University and Boston Medical Center. Special thanks to our...director of the Center for Regenerative Medicine at Boston University Medical School. Darrell’s research focuses...Chapter 1: Introduction to Darrell Kotton and the Boston University Center for Regenerative Medicine. Darrell...Center, and the Pulmonary outpatient clinic, all at Boston Medical Center. In addition he conducts basic stem...

...my training, I dove straight into my capstone project. A capstone project is a major project given to ...Figure 1: Addgene's summer interns presenting their capstone projects during the Summer Intern Poster Presentation... scientist. But I expected something wrong. My capstone project was based on plasmid cloning and I, the... Once everything was confirmed, I worked on my capstone project report and presentation, during the final...

...you questions. Remember that people like a good story and science involves characters – in this case you...or other places online, and consider how those stories are being told. Twitter can be a good way to follow...surprised to find that his friends and family finally understood why he cared to study mice on some distant island...the East Coast, there’s programs at both MIT and Boston University. At Addgene we're committed to scientific...sharing – both your plasmids and your research stories. Consider practicing communicating your research...

...resistance gene (missing the first two codons). Two stop codons are inserted between the truncated gene and...ligated into the MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the...Toolkit for constructing fusion proteins with customizable N- and C-terminal modules   Thanks to the lab...Technologies. The MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists...bound to the mRNA since they are tethered AFTER the stop codon. Thus a non-translated mRNA will appear yellow...: 10.1126/science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector...LacZ, puromycin or CreERT2 can be combined with custom-cloned vectors in a Multisite Gateway reaction ...

...not necessarily represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The ...dabbles in science policy, public outreach, and restorative justice.  Follow her on twitter @sung_vanessa...to join the public conversation and to tell the stories behind our research.  That's why I marched for ...Michigan, where programs like the Great Lakes Restoration Initiative are at risk of major cuts and where...behind these two fingers the ninety-nine cubits of Aristotle, nor would you mention my small error and at the...second and that it is our duty to tell our science stories in a manner that encourages open discourse with...

...IP and ChIP assays. Tag 96 genes at a time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP...first step of optimization, we started off by using custom synthetic two-part cr/tracrRNAs for CRISPR experiments...tagging, pick a crRNA that cuts 8-15 bp downstream of Stop codon; for N-terminus tagging, a crRNA cutting 8...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...near the insertion site (within ~8-15 bp from the stop codon for C-terminus tagging) GC content of the ... hands, delivering Cas9 protein in complex with custom synthetic cr:tracrRNAs (csRNP) is the most efficient...