We narrowed to 435 results for: Trac

1. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

   Type

   Blog Post

   Published

   Nov. 29, 2018, 1:53 p.m.

   ...and intergenic regions containing the putative tracrRNA into a plasmid and expressed it in an E. coli ...

2. Designer PUF Proteins for Any RNA Target

   Type

   Blog Post

   Published

   June 17, 2014, 7:57 p.m.

   ...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

3. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

   Type

   Blog Post

   Published

   Jan. 23, 2014, 3:23 p.m.

   ...efficiency, simply stopped working. They finally traced the problem back to the endonuclease. “It’s just...

4. Let There Be LITE Plasmids

   Type

   Blog Post

   Published

   Nov. 21, 2013, 3:56 p.m.

   ...light dose and is fully reversible after light retraction. Finally, because light can be applied non-invasively...

5. Editor's Choice, September 2016

   Type

   Blog Post

   Published

   Oct. 7, 2016, 4 p.m.

   ...International Airport listening to the Lion King soundtrack and writing this post, it is my pleasure to announce...

6. Top 10 Open Science Developments of 2013

   Type

   Blog Post

   Published

   Dec. 19, 2013, 3:46 p.m.

   ...as the open science movement continues to gain traction. One way to consider where the open access (OA...

7. Six Spooky Science Stories and Halloween at Addgene

   Type

   Blog Post

   Published

   Oct. 31, 2019, 1:06 p.m.

   ...larvae. The adult worms develop in the digestive tract and release eggs in the bird feces, which get eaten...

8. Seeing Red: Simple GFP Photoconversion

   Type

   Blog Post

   Published

   Aug. 4, 2015, 2:30 p.m.

   ...Green to red photoconversion of GFP for protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038...

9. IDP and your PI: A Roadmap for Career Planning and Personal Development

   Type

   Blog Post

   Published

   July 21, 2015, 2:30 p.m.

   ...PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong Science Career Path...

10. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type

    Blog Post

    Published

    Feb. 18, 2015, 3:09 p.m.

    ...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...

11. Tag Your Favorite Yeast Genes with Ease

    Type

    Blog Post

    Published

    Nov. 19, 2013, 2:37 p.m.

    ...native conditions and allow scientists to not only track the abundance, movement, and localization of individual...

12. What's New in CRISPR - March 2020

    Type

    Blog Post

    Published

    March 10, 2020, 1:15 p.m.

    ...fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells that express both the dCas9 construct and...

13. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type

    Blog Post

    Published

    Nov. 27, 2019, 2:03 p.m.

    ...Combinatorial strategies can also be used for retrograde tracing: visualizing neurons that received information ...

14. Future of Research Conference - Remarkable Opening Session

    Type

    Blog Post

    Published

    Oct. 7, 2014, 3 p.m.

    ...that can be taken now to solve some of the more “tractable” problems.  We must better define the postdoc ...

15. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type

    Blog Post

    Published

    July 29, 2014, 6:16 p.m.

    ...everywhere to get a clearer picture of that intracellular world, even as the Davidson lab prepares to ...

16. In Living Color: The Skinny on In Vivo Imaging Tools

    Type

    Blog Post

    Published

    March 27, 2014, 5:14 p.m.

    ... and intensity allows for optical labeling and tracking of proteins, organelles, and living cells in a...

17. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type

    Blog Post

    Published

    Feb. 13, 2018, 2:51 p.m.

    ...permeabilize cells rather than to rely on nuclei extraction. They report that this change offers a significant...

18. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type

    Blog Post

    Published

    Dec. 13, 2017, 3:20 p.m.

    ... on the Addgene Blog Use Apps to Save Time and Track Your Experiments Use video to share your science...

19. Tips for Improving Your Next Manuscript

    Type

    Blog Post

    Published

    Nov. 22, 2016, 3:30 p.m.

    ... discussed individual sections of a paper, the abstract, figures, introduction, etc. so as to thoroughly...

20. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type

    Blog Post

    Published

    June 4, 2020, 1:22 p.m.

    ...main causes of degradation during plasmid DNA extraction and purification from E. coli is the presence...

21. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type

    Blog Post

    Published

    Feb. 16, 2021, 2:15 p.m.

    ...a desk, although there’s often technical career tracks for PhDs who want to stay at the bench. So if you...

22. Hot Plasmids - October 2022

    Type

    Blog Post

    Published

    Oct. 6, 2022, 1:15 p.m.

    ...of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse...

23. Management for Scientists: Giving Feedback

    Type

    Blog Post

    Published

    Feb. 6, 2015, 2:46 p.m.

    ...back to the issues if the conversation gets off track or becomes emotional. Don’t let things fester,...

24. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    Published

    Aug. 19, 2014, 3:39 p.m.

    ...whistles to your construct. If you are planning to track your protein in the cell or purify it – and you ...

25. 10 Ways to Share Your Science!

    Type

    Blog Post

    Published

    May 13, 2014, 8:34 p.m.

    ...it another way. You’ll know you are on the right track if they start asking you questions. Remember that...

26. The Stingy Scientist: How the Baby Gel Box Was Born

    Type

    Blog Post

    Published

    April 15, 2014, 3:11 p.m.

    ...saving freezer space).  The procedure becomes less attractive when noting that one step involves boiling the...

27. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...generating protein machines that can extend and retract over many microns in response to pH changes without...

28. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    Published

    Sept. 21, 2023, 1:15 p.m.

    ...solutions will help get your experiments back on track.  One final note — this post focuses on the species-on-species...

29. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...

30. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type

    Blog Post

    Published

    Sept. 17, 2020, 1:15 p.m.

    ...could keep up with demand and keep new projects on track to succeed at the same time. I began approaching...