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  1. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...Marcy Patrick CRISPR-Cas9 FAQs Answered! By Caroline LaManna Plasmids 101: What is a plasmid? By...  Caroline LaManna...

  2. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...about picking a good mentor, and begin considering career options early. Tune in for great advice on all ...Looks to Improve the Research Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab ...

  4. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... Addgene Outreach Scientist and fellow blogger Caroline LaManna put it well: "Wow, custom mutations in...CRISPR news was circulating. Scientists at North Carolina State, in the labs of Chase L. Beisel and Rodolphe...

  7. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...with a blog post with creative ways to continue career development virtually. 6. Quantifying DNA? Here...for extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular Biology...

  8. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ... to try this at home in their own lab: be very careful with the type IIS restriction endonuclease of choice... PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision Cloning...

  9. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...covered in a future post in this series). Members carry on back-channel or side conversations within the...and suggestions. Consider offering rewards (gift card, afternoon off, etc.) Act on information to make...

  11. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ... that your request is ready to order. An “Add to Cart” button will appear with the request in your Addgene... be charged once you have added the prep to your cart and checked out.  Prices for Packaged on Request...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...genomic loci of interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector...sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...

  14. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ..., R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating that ...and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity...

  15. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...dendritic cells. These genetic tools were pivotal in carrying out my studies, and getting them from collaborators...has since studied and worked around the world, carrying out her undergraduate work at the University of...

  17. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    .... Writing is the cornerstone of any scientist’s career. We use writing to communicate our findings and...consisted of graduate students, post-grads, and early career scientists. Over the course of the class, the moderator...

  18. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ... stone into the next professional stage of your career by connecting you with people of similar interests... research question that people (especially you!) care about. Have you critically evaluated your plan...

  19. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...wall synthesis Bactericidal 100-200 ug/mL Carbenicillin beta-lactam Inhibits cell wall synthesis Bactericidal...optimal efficiency. Beware of satellite colonies! Carbenicillin is more stable than Ampicillin and can be used...

  20. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...Author: James Atmos (2). Many CRISPR/Cas moieties carry out these functions using slightly different Cas... Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Jan;  ...

  21. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth ...Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype ...

  23. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ... MBP tag can be removed using Xa protease. The carboxyl-terminus of the fusion protein includes an AviTag...for affinity purification (see plasmid map and cartoon below). Including biotin in your culture and inducing...

  24. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ... new particles. As a bonus, this increases the carrying capacity of the viral vector, increasing both ... possible, just to increase the viral vector’s carrying capacity. In order to allow the viral vectors ...

  25. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...pigments primarily found in plants in the order Caryophyllales, such as cacti and beets. Betanin belongs to... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy...

  26. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...LTRs). After recombination, one resulting plasmid carries the transgene of interest (aka the viral genome...picking the smaller colonies which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed...

  27. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now... below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only the...

  28. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...compromising on the relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science ...Story by Angela Saini "This book is a smart and carefully presented case for better more diverse science...

  29. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check out this video....Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...

  30. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...cell on their way to the nucleus where their gene cargoes can be expressed. The bottom line is that a lot...particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating your...
Showing: 241 - 270 of 523 results