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... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology...

...Compiled Info on New Plasmids Review the CRISPR Field with Our CRISPR Guide Pages ...

...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

...culture in the present day Cell culture is a widespread tool used in the fields of oncology, virology...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...

...Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant Populations...Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled Libraries to Generate Mutants ...

... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic...

...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...undergone recombination. This default state was disproportionately expressed by a majority of neurons, limiting...

...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology...

...PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes...Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org...

...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...

...each serotype also exhibits distinct patterns of spread from the injection site it is advisable to try ...off-target expression making it easier to determine the spread pattern around the injection site. Test the promoter...

...without telling you anything about outliers or data spread within those data sets. Measures of dispersion...allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve) or skewed...

...by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience community like wildfire...identification of opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type...

...analyzing qPCR data, most with plug-and-play Excel spreadsheets available for easy analysis. These useful tools... vol. 1160, DOI 10.1007/978-1-4939-0733-5_3, © Springer Science+Business Media New York 2014Bustin S; ...

...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute to... theses chimaeras to produce all-iPSC derived offspring demonstrates the ability of the iPSCs to form ...

...eBook webpage.  More resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition...

...for the upcoming wintertime festivities! Help us spread some cheer by snapping a photo of you and your ...

...photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your ...

...seminal” studies for various categories, including CRISPR gene-editing, the foundations of synthetic biology...

... crispr...

...a stick is dragged through the first streak and spread over the second quadrant. The process continues...

...showcases the five main topics of the blog: Plasmids CRISPR Viral Vectors Fluorescent Proteins Science Careers...

...mammals), cloning techniques, and experimental use (CRISPR, viral production, RNAi, protein expression etc...

...submission includes: Images that clearly show viral spread and cellular tropism* (min. 300 dpi, PNG format...

... to use the expertise he’s developed as well as CRISPR-based gene repression tools to find and test genes...

...chart below, we are making great progress toward spreading the Addgene community into developing regions ...

...things like DNA synthesis, Golden Gate cloning, and CRISPR. The yeast synthetic genome project and constructing...

...disease once limited to small pockets in Africa has spread to urban areas and across the ocean. A nurse in...

... distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids. Our most...

... lentiviral preps for COVID-19 research, pooled CRISPR lentiirus libraries, pooled barcoding libraries...

...the Inntag Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein of Interest ...

...University of Toronto, created The Addictive Brain to spread awareness about how scientists utilize tax-payer...

... a wide variety of programs, including fall and spring lecture series, science cafes, and Reddit AMAs....

...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...

...Needed to Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual...

...Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide Screening Browse 28 ...

... of the topics they've recently covered inlcude CRISPR, mosquitoes, allergies, & more! We briefly answer...

...ant’s death, the fungal fruiting bodies being to sprout out of the ant’s body. The fruiting bodies then...

...represent the irradiated area. Photoconverted GFP spreads through the cytosol via cytoplasmic strands. Image...