960 results

Binning Singletons is a networking and mentoring program for conference attendees. Learn about the program and how you can participate as a mentor or mentee.

Feb. 18, 2020, 2:15 p.m.

Help us pick a final name for our mascot's new virus friend!

Feb. 13, 2020, 2:34 p.m.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Feb. 11, 2020, 2:15 p.m.

Sarting a journal club? Read our guide to the logistics of forming a journal club and find different formats to hold the journal club discussion.

Feb. 6, 2020, 3 p.m.

Chromoproteins are a subset of the fluorescent protein family. They give strong color in visible light and can be used for molecular biology experiments.

Feb. 4, 2020, 2:15 p.m.

Why is research irreproducible and how can we improve reproducibility? One way is by using Registered Reports where peer review occurs before results are known paper.

Jan. 30, 2020, 2:15 p.m.

Read about the iGEM IISER Bhopal team and their work developing a cold tolerant E. coli using specialized chaperones.

Jan. 28, 2020, 2:35 p.m.

Get an inside look into the software development process. at Addgene.

Jan. 23, 2020, 2:45 p.m.

The Open Repository of CRISPR Screens is a resource to increase the accessibility of CRISPR screen data. Learn how to use this for your research.

Jan. 21, 2020, 2:30 p.m.

Congratulations to the Deck the Lab winners on their creative holiday lab decorations!

Jan. 17, 2020, 3 p.m.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Jan. 16, 2020, 2:18 p.m.

Overwhelmed by too many papers to read? Find four ways to search for and save relevant papers.

Jan. 9, 2020, 1:45 p.m.

Addgene's mascot, Blugene, recently got a make-over, and along with that, they have a new friend. Help us name Blugene's virus friend.

Jan. 7, 2020, 1:55 p.m.

Check out the top ten blog posts on the Addgene blog from 2019. Topics include CRISPR, fluorescent protein, plasmids, and more.

Dec. 31, 2019, 2:26 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including optogenetics AAV, control AAV, biosensor AAV, and more.

Dec. 19, 2019, 2:40 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for CRISPRi in Burkholderi, DNA and RNA editing, epitope tagging, and Cas9/gRNA ribonucleoprotein delivery.

Dec. 17, 2019, 2:37 p.m.

Get a behind-the-scenes look at Addgene's new search engine.

Dec. 12, 2019, 2:22 p.m.

Learn about new plasmids at Addgene including CRISPRa in bacteria, voltage indicators, CRISPR knockout library for mice, and lipoprotein tools.

Dec. 10, 2019, 1:58 p.m.

Get a behind-the-scenes glimpse of the design behind Addgene's new homepage and mascot update.

Dec. 5, 2019, 1:49 p.m.

Find plasmids more easily with the new search feature on Addgene's website! Now, you can set filters to narrow down your plasmid or viral service search.

Dec. 3, 2019, 11:38 a.m.

There are many method to identify protein-protein interactions. Learn about different method of proximity labeling based on biotin-streptavidin interactions.

Dec. 2, 2019, 2 p.m.

When using AAV for precise neuronal targeting consider AAV tropism and administration route, cell-type specific expression, and combining different approaches to increase targeting specificity further.

Nov. 27, 2019, 2:03 p.m.

Working across teams can lead to break downs in communication. Learn how removing jargon, adding context, and creating a lexicon document can improve cross-team communication.

Nov. 21, 2019, 1:53 p.m.

Opto-nanobodies (OptoNBs) are nanobodies that can be controlled by light. Learn how you can use OptoNBs to purify proteins or to modulate cell signaling.

Nov. 19, 2019, 2:08 p.m.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

Nov. 12, 2019, 2 p.m.

Learn about some things you can do to help make your experimental methods more reproducible.

Nov. 7, 2019, 2:18 p.m.

Learn about some newly developed Cas proteins and how to choose a Cas protein based on site accessibility, specificity, and sensitivity.

Nov. 5, 2019, 1:28 p.m.

Read six spooky science stories and learn about Halloween traditions at Addgene.

Oct. 31, 2019, 1:06 p.m.

All living cells possess a bioelectric state. Learn about how bioelectricity can advance research in birth defects, regeneration, medicine, and synthetic bioengineering

Oct. 29, 2019, 2:52 p.m.

There are many thing to consider when designing a scientific poster: font size, layout, background, and color. Learn more about poster design in this blog post

Oct. 22, 2019, 12:20 p.m.

Learn how FLEx and optogenetics combined can be combined to control gene expression with high spatial and temporal resolution.

Oct. 17, 2019, 12:28 p.m.

It's #AddgeneGoesViral week on Twitter! Visit our Twitter page for discussion on viral vectors, their applications, and what's new from Addgene's viral service.

Oct. 14, 2019, 1 p.m.

The HA frankenbody combines optimized elements of an antibody probe with a fluorescent protein and can be used to easily visualize single molecules and newly formed proteins. 

Oct. 10, 2019, 12:41 p.m.

Announcing Addgene's AAV Data Hub! The Data Hub is an open platform for scientists using AAV to share practical information about their experiences.

Oct. 8, 2019, 12:33 p.m.

Find some of our favorite science and non-science books and learn about the Addgene Book Club.

Oct. 3, 2019, 12:47 p.m.

Uncertain about using LinkedIn as a scientist? Find seven tips for leveraging your LinkedIn profile for your career.

Oct. 1, 2019, 1:21 p.m.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Sept. 26, 2019, 12:50 p.m.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Sept. 24, 2019, 12:55 p.m.

Learn about some ways to study behavioral neuroscience with viral vectors using behavioral tests for locomotion, anxiety, and depression.

Sept. 17, 2019, 12:40 p.m.

Learn about new and upcoming viral vectors from Addgene including our new trial size AAVs for serotype testing, calcium and GABA sensors, and more!

Sept. 12, 2019, 1 p.m.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Sept. 10, 2019, 12:56 p.m.

Stop waiting for your MTA approval to go through & talk to your technology transfer office. Find tips for working with your university TTO as a researcher.

Sept. 5, 2019, 1:06 p.m.

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

Sept. 3, 2019, 1:15 p.m.

Are you going to present or attend a scientific conference soon? Find tips for success as a presenter and attendee.

Aug. 29, 2019, 12:50 p.m.

Are you looking to make your office more eco-friendly? Read this blog post to learn what we've been doing to make our office more sustainable!

Aug. 27, 2019, 1:08 p.m.

Designing a cloning experiment? Read this blog post to learn about different positive and negative selection strategies you can take to find the clones you're looking for.

Aug. 22, 2019, 12:43 p.m.

If you're new to behavioral neuroscience, this article will walk you through how to standardize your experiments and chose viral vectors appropriate for your experiment.

Aug. 20, 2019, 12:31 p.m.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Aug. 15, 2019, 12:30 p.m.

Zebrafish larvae is a great model for studying development and embryogenesis. Learn more about genetic tools for zebrafish in this blog post.

Aug. 13, 2019, 12:58 p.m.

New to peer-review? This peer-review checklist takes you through different steps and things you should consider while writing a peer-review. Remember, be critical and kind and advocate for open science.

Aug. 8, 2019, 1:19 p.m.