We narrowed to 746 results for: TRI

... functions they may induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

...Addgene has distributed over 100,000 CRISPR plasmids. But that’s not our only job - we strive to also give...

... experiments to the PIs. Remote meetings can be tricky, and it takes extra effort to make sure everyone...everyone in the meeting has an equal opportunity to contribute. Using video conferencing tools like Zoom or ...

...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...open access and available without any licensing restrictions.    As CRISPR technology continues to develop...

... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

...channelrhodopsins (KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors...not just because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated...

...    sdAbs in research These advantages have contributed to sdAbs becoming valuable tools for diverse ... approved in 2018, with many others in clinical trials (Jin, et al. 2023). In addition to therapeutics...

...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...separated by CaM and M13. When calcium binds, it triggers CaM/M13 interaction that increases FRET transfer...

...for sale, and photos of the Agar Art competition entries were displayed in a gallery on the second floor... to find out more about our free deposit and distribution service! Did you see a must-have plasmid during...

...RFP, no FP). The vectors each contain two SfiI restriction sites with different overhangs depending upon...Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as a nonviral...

...entire meeting, Monday's talks alone were worth the trip. Dr. Dana Carroll's excellent keynote address was...genome engineering tools – both deposited and distributed – have changed so dramatically in the past 4 ...

...one step involves boiling the glass powder in 50% nitric acid, but I never let that stop me. I only had ...glass slide using only surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t...

...to the antibiotic that remains intact. Tips and tricks from the bench Use fresh stocks. Most antibiotics... Marcy Patrick...

...fragments that recognize the endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, ...https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application...

...HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to ...cells may be particularly useful due to their intrinsic properties. HEK-293 cells are tumorigenic, ...

...This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created...don’t use SnapGene yet, you can download a free trial. If you notice that a feature is missing from our...

... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...dialogue specifically about the project and contributions, rather than getting defensive or getting into...

...mL Amp instead of 100 μg/mL Amp), or using more nutrient rich media can improve growth and DNA yield. Test...determine if your sample has recombined. Choose a restriction enzyme that will give you different band patterns...

... dark matter and so the lab organized a sampling trip to Yellowstone National Park (YNP). Extremophiles... another Quake Lab member joined for half of the trip. Preparations ahead of time included contacting ...

...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...copies per cell per color, following a Poisson distribution. To overcome the limited expression levels of...

...fantastic fodder for science communication. The trick is to find one or two roadblocks that sparked creativity...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...

...suggest topics you’d like to see on the blog or to contribute ideas for your own guest posts by e-mailing us...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...

...was founded in order to store, QC, curate, and distribute them all in the name of making it a little bit... Marcy Patrick...

...submissions from 99 universities and foundations in 21 countries. “Over $600,000 worth of cutting-edge technologies...with one form of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different...

...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel... the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD...

...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...workshop. Future workshops for Addgene Addgene strives to be a great resource for the research community...

... This guest post was contributed by Marco Straccia, an Associate Professor at University of Barcelona...governed how this game was played. These rules restricted which cards could be played when and, depending...

..., there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families...

...help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi...

...“leaf” with similar permeability of water and nutrients and colonization opportunities for bacteria. To...the control of promoters responsive to water or nutrient levels can help him determine whether the artificial...

...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...built-in whiteboard, in which participants can contribute and sketch on the board without leaving Zoom....

...Apoptosis is a highly controlled process that is triggered by internal and external signals such as developmental...new protein-protein interaction sensor based on tripartite split-GFP association." Scientific reports 3 ...

...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...

...This post was contributed by Kelly Hills, founding bioethicist of Rogue Bioethics. On November 25, the...animal models, and several stages of human clinical trials. This model also demonstrates that when we are ...

...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...

...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact...

...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors...

...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...embargoed while the research is ongoing, but all registries do require that research plans become public ...

...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

...This guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference ...employer that your past work has prepared you to contribute at a high level to their team.  When you're writing...

...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...

...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....you might need different plasmids. Harvard’s DRSC/TRiP Functional Genomics Resource Center has various ...

...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you... mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407–...

...This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... best way to find a system that does is through trial and error, and by asking for tips from others. Comment...