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Showing: 401 - 450 of 728 results

  1. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...copies per cell per color, following a Poisson distribution. To overcome the limited expression levels of...

  2. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...mL Amp instead of 100 μg/mL Amp), or using more nutrient rich media can improve growth and DNA yield. Test...determine if your sample has recombined. Choose a restriction enzyme that will give you different band patterns...

  3. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...fragments that recognize the endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, ...https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application...

  4. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to ...cells may be particularly useful due to their intrinsic properties. HEK-293 cells are tumorigenic, ...

  5. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created...don’t use SnapGene yet, you can download a free trial. If you notice that a feature is missing from our...

  6. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...dialogue specifically about the project and contributions, rather than getting defensive or getting into...

  7. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...fantastic fodder for science communication. The trick is to find one or two roadblocks that sparked creativity...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...

  8. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...suggest topics you’d like to see on the blog or to contribute ideas for your own guest posts by e-mailing us...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...

  10. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...submissions from 99 universities and foundations in 21 countries. “Over $600,000 worth of cutting-edge technologies...with one form of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different...

  11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel... the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD...

  12. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi...

  14. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...Apoptosis is a highly controlled process that is triggered by internal and external signals such as developmental...new protein-protein interaction sensor based on tripartite split-GFP association." Scientific reports 3 ...

  15. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...

  16. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...This post was contributed by Kelly Hills, founding bioethicist of Rogue Bioethics. On November 25, the...animal models, and several stages of human clinical trials. This model also demonstrates that when we are ...

  17. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...built-in whiteboard, in which participants can contribute and sketch on the board without leaving Zoom....

  18. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ... This guest post was contributed by Marco Straccia, an Associate Professor at University of Barcelona...governed how this game was played. These rules restricted which cards could be played when and, depending...

  19. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...workshop. Future workshops for Addgene Addgene strives to be a great resource for the research community...

  20. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families...

  21. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...

  22. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

  23. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455 19 Coelenterazine None Yes Luminous ...Gaussia princeps) 480 20 Coelenterazine None Yes Metridia longa 480 24 Coelenterazine None Yes North American...

  24. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

  26. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

  27. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

  28. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

  29. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact...

  31. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you... mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407–...

  32. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

  33. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...This guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference ...employer that your past work has prepared you to contribute at a high level to their team.  When you're writing...

  34. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors...

  35. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...

  36. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... best way to find a system that does is through trial and error, and by asking for tips from others. Comment...

  37. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...top of pre-made plates, make sure it is evenly distributed and allow sufficient drying time before use. ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

  38. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...mutations at off-target sites. Christina Mork contributed to the update of this post. Thank you to our ...

  39. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering... science, these tools sometimes have limited distribution. We hope that will change with Addgene’s expansion...

  40. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...gene or in multiple genes, all of which seem to contribute to a particular disease state. You could use ...

  41. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...channels that are responsible for maintaining the nutrient and ionic balance of the cells,” says Dr Sangeetha...keratinocytes, which release inflammatory mediators that trigger the activation of dendritic cells, macrophages,...

  42. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...support team, is a safer bet than one from a large distributor that may not have direct experience with the ...

  44. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular... depend on fluorescence intensity or protein distribution. Therefore, photostability measurements can ...

  45. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...Greek for “self-eating”) is induced by a lack of nutrients and targets cytosol and organelles to the vacuole... have fused with the vacuole or a lysosome are trickier to identify because their contents can be at various...

  47. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...of endogenous proteins (notably, BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules...

  48. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

  49. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

  50. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...
Showing: 401 - 450 of 728 results