Skip to main content

We narrowed to 330 results for: ARIA;

Showing: 161 - 180 of 330 results
  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly diving...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...
  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...
  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...however, that is generally not true. Because of the variations in fluorescent signals, and how the machine reads...SSC, as briefly alluded to above. There’s many variations in how one can gate, select, and analyze data...
  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s, scientists...
  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...
  6. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is... Good luck! This post was originally written by Maria Soriano-Carot in September 2016 and was updated ...
  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...
  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange... mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow...
  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and 100 times... and cytotoxicity better than the 50x and 100x variants. But those super-high efficiency constructs are...
  10. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred to as Cas9) and a single stranded... the nucleus, and several codon optimized Cas9 variants have been created in an effort to increase translation...
  11. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...mutated genes. Next, the authors explored how these variants of p53 shape chemosensitivity and mechanistic ...provides a HiBiT-enabled framework for quantifying variant expression with a minimally disruptive tag. Find...
  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ...
  13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...vector for this step may save time as it reduces variability that may be encountered with DIO/FLEx vectors...rare for a methods section to include all of the variables that need to be accounted for (i.e. serotype, ...
  14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...integrations per cell, and my stable cell pool had wildly variable Cas9 expression (Figure 1). This is a problem ...monoclonal lines (Figure 2) and I was surprised at how variable Cas9-expression was across the different cells...
  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now the most popular...any cloning at all! As mentioned above, several variants of these tags have been developed over the years...
Showing: 161 - 180 of 330 results