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We narrowed to 171 results for: Apple fluorescent protein

Showing: 161 - 171 of 171 results

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...step: protein degradation, incomplete lysing/insoluble proteins, and protein aggregation. Protein degradation...isolating proteins from a specific compartment. If your proteins are nuclear- or DNA-binding proteins, you ...two types of gradients. Protein gradients use dilutions of your sample or protein of interest to understand...  Figure 1: A protein gradient. The density of the bands, which increase as protein concentration increases...in order to release the proteins from their binding. If you’re seeing protein aggregation, you may want...you need very accurate protein measurements, consider using an unstained protein ladder, which will run...reversible protein staining immediately after your transfer. If you are expecting low amounts of protein, make...

  3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...of DNA to make RNA, translation of RNA to make proteins. That's central to everything that goes on inside...regulation of gene expression including growth or fluorescence. There are a few examples of engineered RNA ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell... have been completed. Therefore, to be ready to apply for a Group Leader position, key first author manuscripts... project. Furthermore, Group Leader position applications require 3 letters of reference- the graduate...informal recommendations, more positive grant application and manuscript reviews, etc. You'll also meet...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...for all applications, such as protein isolation from serum. In this case, Protein A and Protein G will ...absence of a protein, determine if a protein is up or downregulated, examine a protein’s stability or ...Hjelm, 1972, Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose...support while unbound proteins are washed away.  While both Protein A and Protein G have an affinity for...compatible with either Protein A or G.  While commonly used, Protein A and Protein G are not suitable for...of the target protein or interfere with protein interactions of interest. Tagged proteins are typically...modifications, or study how a target protein interacts with other proteins or nucleic acids. Read on to learn...

  6. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...molecule, the antibody binds to a protein, and then voila! Wherever your protein of interest is, a signal will...molecules to identify each protein of interest, this gel-based method identifies proteins solely by weight and... isn’t giving you enough signal to detect your protein of interest, it may be time to look at the indirect...antibodies. While a primary antibody binds to the protein of interest, a secondary antibody binds to the ...signaling molecules associated, indirectly, with each protein of interest.    Figure 1: The direct ... molecules are represented in light green; and proteins are represented in orange. Created with BioRender.com...three, secondary antibodies are indicated when the protein of interest is found in low amounts or if the conjugated...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc...BBB via interactions with a similar protein called LY6C1. This protein is expressed in both BALB/cJ mice...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While... it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019; Huang et al...capsids that rely on interactions with those two proteins to cross the BBB are often not effective in NHPs... selected for its ability to bind to the LY6C1 protein in order to reduce strain-specific tropism in mice... W. L., & Wilson, J. M. (2019). The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you...When assembled, the four chains make a Y-shaped protein that can be divided into the Fragment Crystallizable...the fragment could bind not only the endogenous proteins you are trying to block but your primary antibody...the antibody you do and don’t need, and how the protein will interact with other components of your experiment... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...pre-form primary-secondary antibody complexes before applying them to samples, such as when performing IHC in...can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are the same species...

  10. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...components including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with...with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array...Zalatan’s lab used an approach to identify known proteins involved in the activation of transcription including...including transcription factors, phage proteins, and RNAP components (Dong et al., 2018). They found a...dCas9 variant fused to bacterial enhancer binding protein required for the activation of sigma-54 type promoters...glycosylase inhibitor and degradation tag on the fusion protein to limit the activity of the system as a whole....tools available for bacteria and some of the applications for which they’ve been used. The beginnings ...

  11. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...unreliability: cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently...atlases/literature and is unrelated to the target protein, we categorize the antibody as having non-specific...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...produced from a new set of animals), and wrong application (different experiments/conditions can change ...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...secondary antibodies, allowing us to only image autofluorescence i.e., background noise. We then compare the...
Showing: 161 - 171 of 171 results