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We narrowed to 534 results for: CAR

Showing: 161 - 180 of 534 results

  1. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ..., to congenital disorders. A series of studies carried out on an isolated population in Sweden found that...that decreased their likelihood of death from cardiovascular disease but increased their risk of death from...paternal grandmothers early food supply influenced cardiovascular mortality of the female grandchildren." BMC...Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined by nutrition...

  2. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...www.FinishYourThesis.com and is one of many posts in our career series.  “The only healthy communication style ...into learning opportunities as you are advancing career, you will become the independent, assertive, and...learn more about assertiveness skills is Dale Carnegie’s book “How To Win Friends and Influence People...Management for Scientists" series More science career articles on our blog Read Joanne Kamen's Post ...

  3. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...their 30s who have quit their academic science careers in order to head to Ireland and take a chance as...product. It was a small project, not a full-blown career, but it was enough to start us down the entrepreneurial...started things up for real: bank account, business cards, incorporation, laboratory lease, etc. I still flip...allowed us to really analyze what we want out of our careers: to build something new and beautiful, to engage...

  4. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...ωRNA) (Figure 2), but TnpB is small enough to be carried by viral vectors (~400 amino acids, less than a...tracking cell lineage, different applications require careful selection or design to ensure reliable readout ...Libraries are available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible...have broad tissue tropism, including skeletal and cardiac muscle, airway epithelial cells, liver, CNS (limited...

  5. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered... vivo, in vitro, ex vivo CRISPR screens can be carried out using a few different methods — in vivo, in...outside a host cell’s genome. In this state, AAV cargo can be stably expressed in non-dividing cells for...

  6. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ... homologous recombination events: The plasmid carrying the desired genetic modification integrates into...ShadowR is used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is ... a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for... New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial Genomes. Applied...

  7. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...your LinkedIn and your resume and prepare business cards. You might not consider being a student or postdoc...postdoc a “business,” but having business cards is a good way to share your information quickly. During the...meet ten new people each day. Exchange business cards. When you’re done networking for the day (or whenever...have a small break), write down some notes on the card so you remember who they are and what you’ve talked...

  8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...ICEBs1) system. ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes, like Mobile-CRISPRi...from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined suite of tools for ...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...to hold up your poster, and pack a few business cards to tack up next to your poster. Arrive early: You... presenter after the session. Exchange business cards or jot down their name and affiliation from the ...the innate immune system, and is interested in a career in science communication.       Resources on the...of the Addgene depositor community Learn about careers at Addgene ...

  10. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...While CRISPR is certainly not the first means to carry out so-called “forward genetic screening experiments...are your cells derived from? Currently, Addgene carries CRISPR libraries that target mouse, human, fly,...genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout, activation,...or a specific class of genes? Addgene currently carries several genome-wide CRISPR libraries and a selection...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...school district that has a lot of parents in STEM careers, chances are they’re wondering how they could contribute... led up to my job at Addgene, which is likely a career path none of them had imagined for a scientist....talked about CRISPR’s molecular mechanism, I was careful to keep it relatively simple. I honed the complexity...testing your SciComm skills or are interested in a career in education. Maybe you just like to see the look...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...non-dividing cells, lentiviral vectors can carry much more genetic cargo (8 kb vs 4.5 kb), and they remain popular..., and transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package ...Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...

  13. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ..., each package is opened and the contents are carefully inspected to verify that the samples arrived intact...dedicated and detail-oriented lab team that works carefully to catch mistakes. Each sample that is transformed...shared with multiple people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours... time searching for samples and keeps us from discarding tubes in error. The Addgene lab works diligently...

  14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...scheme very nice at first sight in that Addgene takes care of everything including MTA and shipping. Addgene... scientific societies. Addgene: Throughout your career, you have held research appointments in the US ...different parts of the world in my early research career. I've found that research institutes in different... Caroline LaManna...

  15. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...students, rotation students, joining a new lab at any career stage, learning a new technique while visiting ...rightfully so! They can provide you with high-level career advice, open doors for you, and teach you valuable...identify a ‘scientific mentor’: someone who provides career advice and offers direction on your scientific ...hasn’t sent you papers or protocols yet, don’t be scared to ask for some! You can email or ask in-person...

  16. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...Read all of our career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch...engaging your audience. You need to make your audience care, to want to know the answer to your research problem...Watch our career videos ...

  17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...and Sota Hiraga found that if a mini-F plasmid carried a specific 700 bp segment of DNA, the plasmid was... without plasmid addiction systems. For example, Cara Morin and James Kaper used the hok/sok system to...be used over prolonged periods. Using a plasmid carrying the hok/sok toxin-antitoxin system, they were ...223.1 (1992): 41-54. PubMed PMID: 1370544. Morin, Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...platform to deliver various cargo to a specific DNA locus by directly fusing the cargo to dCas9.  This technique...next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene.  Validated...CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that have...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...Non-Academic Career Decisions Check Out Our Management for Scientists Series Science Career Options Resources...Addgene.org Learn more About Careers at Addgene ... academic career...

  20. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ..., if you know what you need. Most often, early career scientists aren’t trained to have these types of... and not on the financial aspects. Money often carries an emotional weight. Therefore, too much focus ... a pathology microtome/cryostat, then making a careful decision on the condition and availability of the...XpDesignsMGNT        Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental...
Showing: 161 - 180 of 534 results