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We narrowed to 520 results for: EMB

Showing: 161 - 180 of 520 results

  1. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...This grows our relationship with our community members. We also find that, as a nonprofit, we attract ...is strength in diversity and in empowering team members to take initiative and give their best As we continue...Research Listen to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn ...

  2. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...an organelle-specific targeting signal or a transmembrane domain, biosensors can be targeted to a particular...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

  3. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its cre-dependent...GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...& Biology Society (EMBC), 4322–4325. https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G....who together developed multifunctional fibers, embedded, and validated simultaneously in the brain and...

  5. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...10,000 users per month in November 2014 to ~30,000 users per month in November 2015. We will continue ...providing recorded interviews with Addgene community members. In addition we’ve experienced incredible growth...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...Tat. Although CPP fusions can translocate a cell membrane, the presence of the CPP may alter protein function...various loxP reporters. In both mouse and human embryonic stem cells, they found very high recombination...neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell ...

  7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination...of CRISPR/Cas9." Cell Stem Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair...Fibrosis Patients." Cell Stem Cell. 13, 653-658 (5 December 2013).    ...

  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ... out these questions ahead of time so that lab members have time to prepare their thoughts. Then at the...Set up a brief bi-weekly meeting where all lab members talk no science. No data presentation will appear...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ...

  9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...differentiation, the Zhang lab transduced human embryonic stem cells with the viral TF library, with each...lentiviral particles and used to transduce human embryonic stem cells. Transduced stem cells were differentiated...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...

  10. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...everyone gets a chance to talk and less vocal team members still have their voices heard. Here's a template...of the agenda, and assign each one using group members’ initials. If appropriate, email this around after...to help keep you accountable for moving along. Remember, many of the more in-depth discussions can happen...

  11. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty...expressing Cas9. As John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you'... running. Both Alex Chavez and John Doench are members of the Addgene Advisory Board. Additional Resources...

  12. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...and improving accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and ... at the Society for Neuroscience conference in November 2023, we hosted our very first professional development...clear writing Let’s use Figure 1 as our example. Remember, our goal is to use words to describe the image...

  13. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...viral envelope, which fuses with the target cell’s membrane, allowing the viral particle access into the cell...inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses can accommodate...of murine leukemia virus DNA depends on mitosis.” EMBO J. 12 (1993) 2099-2108. PubMed PMID: 8491198. PubMed...

  15. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...and names, swapped leadership, and grown to 25 members.  What is Sample6? Sample6 is an enrichment-free...technologies, effectively communicating the advantages embodied, and driving the enterprise value of Sample6.  ...Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's ...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... indirect (secondary) methods. In those cases, remember that a secondary should be used when signal amplification...price point, shipping time, or other factors. Just remember that these are secondary considerations to validation...validated using one or more of the proposed pillars.  Remember, validation does not mean that the antibody is...

  17. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... grow. We are hiring again and getting ready to embark on new exciting projects. Right now we don’t .... We are making an impact.  {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, width='1280', height='720', player_id='7039679868...

  18. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    Published
    Oct. 21, 2025, 1:15 p.m.
    ...Biology (Howarth, 2025). Figure 1: Modular assembly tools, alongside criteria for Click Biology. Created...systems. What are the possibilities for modular assembly using the reactive groups naturally present in... steps. Beyond the lab, these systems can also assemble vaccines, target gene therapy vectors, or lock...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...found at Addgene! Figure 1: Strategy for assembling the barcoded gRNA library pool. (Adapted from...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12...your experiments. Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted...
Showing: 161 - 180 of 520 results