...main decisions to make: 1. Which modification? and 2. Which viral vector should be used? Modifying gene...status, diet, cage enrichment, stress level, origin (vendor or in-house mouse breeding colony) Environment...

...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different...expressed in the neuron, the FingRs bind to the endogenous synaptic proteins allowing them to localize to...

...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...abasic (empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various...efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient enough to hinder adenine...

...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...makes the teller feel heard. I am not always a stupendous listener, but I aspire to  be. If you don’t at...conflicts within a given organization, it can help tremendously to have as much context as possible. When I ...

...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...albumin. Secondary antibodies can also react with endogenous antibodies naturally present in the sample, for...experimental run (Burry 2011). Autofluorescence Endogenous fluorescence of the sample, or autofluorescence...

...You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering...Charpentier E. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012...

... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...to a histone tail to recruit and stimulate the endogenous methyltransferase. The strategy is generalizable... protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited methyltransferase DNMT3A to the...

...improvement in muscle physiological performance.  2. Trans-splicing Overview: For trans-plicing, splice...from one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such as a 77bp ...

...would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next time is ...Vanessa Bullard of Chenery Middle School for their tremendous guidance and feedback. Additional resources on...

...GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then...Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like activity (Hu et al., 1990). We commonly...

...Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that encodes...efficiency is correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et...

... Methods 2009; 6, 343-345. PubMed PMID: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey...specific restriction sites (Type IIS restriction endonucleases) to cleave DNA outside of the recognition sequence...

... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...Table 1) were optimized for efficient binding of endogenous BV.  When imaging NIR FPs, consider using light...

...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...field with the most basic equipment and was a tremendously positive experience. I enjoyed helping people...

...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins...