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... Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA...confirmations like linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene...; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide data...bacterial colonies using one of the detection methods described above can help ensure the isolation of the monomeric... harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi...

...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench ...submission of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don...qualifications to apply for a position.  A job description is usually a wish list and the hiring manager... way to a hiring manager. Learn to confidently describe your strengths in your resume and don't be shy...

...resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant phenotypes (unc-... of lethal mutation co-selection. Only animals rescued for the conditional lethal mutation survive the...experiment, five knock-ins were recovered from seven rescued F1 animals (gel image under worm). Image courtesy...on a plate, other than the parental animal, are rescued progeny, which makes screening extremely rapid....available through Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of...

...Runne-Janczy, a Product Development Manager at eScience Labs, an educational company that creates hands-on...scicomm and part two focusing on what her job at eScience Labs is like. Find all the posts in this series...don’t really know where I came from.” Working at eScience Labs, an educational company that creates hands-on...editing and searching for a job and I stumbled upon eScience by luck, by chance, by complete happenstance. ...d had at Iowa really opened the door for me at eScience.” Stay tuned for part two of Caitlin’s interview...

...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...Cre- or Cas9-mediated recombination. Both primary hESCs and immortalized cells treated with iTOP efficiently...populations. After iTOP treatment, both primary (hESCs) and cultured cells showed up to 70% resistance ... frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that iTOP may...

...for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et ...the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques...www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found here: https...Aptamer base: a collaborative knowledge base to describe aptamers and SELEX experiments. Database, bas006...

...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ... vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...

...separate DNA or transforming cells with green fluorescent protein, these activities are limited to those...Typically, if you want to make a protein – say green fluorescent protein – you first need the DNA sequence that... instructions it needs to produce that green fluorescent protein through transcription and translation...students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can ...Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic biology education kit." Science Advances...

... - MG-2I in this case). The fluorogen is not fluorescent until it binds its protein partner thus forming...which suggests that it could be used with these fluorescent proteins. He et al also compared MG-2I-dL5** ...head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer protein that generates ROS when...Optogenetics Blog Posts Sign Up to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores...Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova...

...various structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains...within the biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach...this condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning ...plasmids containing promoter regions that activate fluorescent protein transcription in response to river contaminants...water samples at specific locations, measure fluorescent signals from the sensor, and send a signal including...

... to our website through the use of alt text to describe images in online spaces. Alt text is used by screen...example. Remember, our goal is to use words to describe the image in a way that helps visually impaired... your alt text, use all of this information to describe the image helpfully. You and your readers are ...learned a lot from listening to others' alt text descriptions, and we’d love to hear yours as well.  More ...write alt text for any image you’re struggling to describe in the results. And many journals accept alt text...

...are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators (GEVI...potential of a cell, the more differentiated and quiescent it is, and the more depolarized (positive) the...anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization...sensor and a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness...

...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent protein...(MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver...different bee species. BTK can be used to introduce fluorescent markers to visualize localization and distribution...division counting (DCDC), relies on the assembly of fluorescent proteins into one bright particle that segregates...

...genes across diverse cell lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9...activation. Synergistic Activation Mediator (SAM) Description SAM uses specially engineered sgRNAs to increase...VPR and SunTag) (Chavez et al., 2016). SunTag Description Rather than using a single copy of VP64 per each...expressed consistently throughout cells. VPR Description VPR fuses a tripartite complex with dCas9 to ... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

...spreadsheet Data Field Description Plasmid Name Use a descriptive plasmid name and match the... in red in the spreadsheet (Figure 2) and are described in Table 1. Some cells have drop-down options ...does this plasmid do?” in <200 characters. A descriptive purpose focuses on the practical application ... Primary Vector Type The vector type should describe how your plasmid is intended to be used. Choose...

...in this post: Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl...New ready-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz...SARS-CoV-2), the virus may acquire mutations to escape these therapeutics. Jesse Bloom’s lab at the Fred...From Starr et al Cell., 2020. (B) Mapping antibody-escape mutations with the yeast display library. From ...characterized them by ELISA, homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After...

...co-express your gene along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow...incorporated into plasmids for research purposes are described below. IRES Elements Translation in eukaryotes...co-express your gene of interest along with a fluorescent protein or selectable marker, it is easiest to...genes at equivalent levels upstream of an IRES fluorescent reporter for easy detection.5  Just released:...efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. Kim...

... FAST system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors...of Cas9 and a photo-dissociable dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa... flanking the DNA-binding cleft of Cas9, the fluorescent domains dimerize and prevent DNA binding in the...chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins can be used to identify specific...gRNA and a dCas9 fusion protein with three red fluorescent tags in U2OS cells. Upon light activation, dCas9...figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated that the...

...until new inflorescences begin to grow. This will also increase the number of inflorescences overall, ...and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (...transformation, you can cut off the primary inflorescence (the flowering portion of the plant) near the.../books/about/Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources...

...Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of the Max Planck Society. Zebrafish (Danio...tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers to observe changes...guest blogger Katherine Rogers from the Friedrich Miescher Lab of the Max Planck Society. Katherine W. Rogers...researcher in Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen...about zebrafish behavioral studies Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...