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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 530 results for: Erf

Showing: 161 - 180 of 530 results

  1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...system for multidirectional control The CRISPR interference (CRISPRi) model consists of a catalytically ...field of metabolic engineering, this is such a powerful and flexible technique that it should be on the...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig...Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments for discovering novel...

  4. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...academia, is someone who visits an institution to perform research there temporarily. The length of stay ...relevant literature for the techniques you will be performing to familiarize yourself. Identify reagents you...

  5. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...cultured following a differentiation protocol before performing single-cell RNA sequencing. The sequencing results...were differentiated and single cell RNA-seq was performed to map TFs to cell fates. (Adapted from Joung ...

  6. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

  7. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...lentiviral vectors from the repository for you. We perform test infections with all of our viral preps and.... We provide enough lentivirus for you to both perform an optimization-scale infection and at least one...

  9. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...term for a range of neat little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More...scientific research is funded by the public and performed by researchers who received their educations from...

  11. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...evaluation of ‌therapeutic immunogenicity is typically performed using traditional 2D assays with immune cell lines...of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016...

  12. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... editing experiments in this publication were performed using a technique called nucleofection, which ...found on our plasmid pages. We encourage any labs performing genome editing experiments to try nucleofection...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...blue and emission spectra are red. Neither is a perfect match to the filter set but the excitation filter...reported brightness measurements. Here, EGFP outperforms the improved folding variants, presumably due...

  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...efficient selection. This is usually achieved by performing a "kill curve" (basically growing cells in various...Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids 101: Antibiotic...

  15. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...underutilized team potential. I often thought that performing a cell culture experiment myself would take less... from technical work. On the other hand, it’s perfectly valid to decide that managing isn’t for you at...

  17. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell... strand to yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur...

  18. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...access neurons, and study their connections, in a powerful way. Using brain structure to understand brain...exhibit retrograde transport and can therefore perform retrograde tracing, but often at the cost of toxicity...

  19. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...generating a monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top ...

  20. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was cloned into a ...frequency within functional light chain sequences. By performing a BciVI digest of the PCR products prior to cloning...
Showing: 161 - 180 of 530 results