We narrowed to 282 results for: FUS
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TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m..... A common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain...Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate to the nucleus where...
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Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria for antibiotic resistance... treatment. This is called a Kirby-Bauer disk diffusion test or Kirby-Bauer susceptibility test (Figure... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest ...light to generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....the construct, dBrainbow includes small epitopes fused to each XFP. Secondary antibodies that target these...epitopes and fluoresce in the same color as the fused XFP can be used to amplify the signal. Imaging endogenous... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....HiFi DNA Assembly (NEB) Cold Fusion Cloning (System Biosciences) In-fusion Cloning (Clontech) Although... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....published and documented by OMICtools. It must be confusing for a newcomer to decide on the right software...of ideas is what makes choosing the right tool confusing and it means that there is no one-size-fits-all... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog PostPublishedDec. 11, 2017, 2:02 p.m....libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency. Listen ... -
Addgene’s New Way to Help You Share Science
TypeBlog PostPublishedNov. 14, 2013, 4:10 p.m....you have a question about culturing iPS cells? Confused by which system to use for genome editing? Know... -
CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog PostPublishedMarch 10, 2015, 2:25 p.m....monoclonal antibody cell line, myeloma cells are fused with activated B cells from the spleen of mice injected... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....substrate for a kinase of interest which is further fused to a bipartite nuclear localization signal (bNLS... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m....might say yes and they might say no but do not confuse networking for a job connection with ongoing relationship... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m.... saturates around 30 uM. For low-abundance GFP fusions, a photoconversion signal may not be observed. ... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....complete their work rather than overwhelmed or confused about what’s coming next. Goal setting and career... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m....Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....hard to find. This made the page look not only confusing, but also made it hard for users to get where ... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three...