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Showing: 161 - 180 of 180 results

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the...

  7. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...

  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...

  12. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers...

  18. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology ...

  20. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...Cas12 is unique because it can process multiple gRNAs under a single promoter, allowing for easy multiplexing...
Showing: 161 - 180 of 180 results