We narrowed to 388 results for: HAL;
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  TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar...
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  Hot Plasmids: Spring 2025TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...
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  Viral Vectors 101: AAV Serotypes and Tissue TropismTypeBlog PostPublishedJan. 16, 2025, 2:15 p.m....transduction of lung cells via airway administration (Halbert et al., 2001). It additionally has tropism for ...-0. https://doi.org/10.1016/j.cell.2025.06.026 Halbert, C. L., Allen, J. M., & Miller, A. D. (2001). Adeno-Associated...
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  Is this the right place for me? 8 tactics for choosing a labTypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....Consider talking to people in the lab across the hall or in the same department as well. What do they ... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork...
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  Scientific Reproducibility - Focusing on Solutions at the Minisymposium on ReproducibilityTypeBlog PostPublishedMay 18, 2018, 7:32 p.m....Pamela Hines (Senior Editor at Science) Edward J. Hall (Professor of Philosophy at Harvard University) ...experiments they’re reading about weren’t changed halfway through in order to chase a positive result. Beyond...
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  Easi-CRISPR: Generating Knock-In and Conditional Mouse ModelsTypeBlog PostPublishedApril 5, 2018, 12:42 p.m....Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...template. Although effective, ivTRT may be more challenging for sequences with complex secondary structure...
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  Viral Vectors 101: Optogenetic ToolsTypeBlog PostPublishedJune 27, 2023, 1:15 p.m....nn1525 Gunaydin, L. A., Yizhar, O., Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...
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  PiggyBac-ing Through the Genome Editing FieldTypeBlog PostPublishedMay 31, 2016, 3:30 p.m....CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...
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  Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m.... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...
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  Antibodies 101: Flow CytometryTypeBlog PostPublishedJuly 20, 2021, 1:15 p.m....then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...
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  Hassle-free 96-well Format Epitope Tagging Using Cas9 RibonucleoproteinTypeBlog PostPublishedJune 28, 2018, 3:01 p.m....Creating epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed ...representing a protein super family). To overcome these challenges, we set out to develop a hassle-free, highly ...
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  Which Fluorescence Microscopy Technique is Best for Me?TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....molecule of GFP. Because red light has approximately half the energy of blue light, two photons of red light...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical...
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  Using AAV for Neuronal TracingTypeBlog PostPublishedAug. 9, 2018, 1:04 p.m....replicates, spreads, causes neurotoxicity, and is lethal if left untreated (for review see Dietzschold et..., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., ...
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  A Case Study of Addgene BioinformaticsTypeBlog PostPublishedMay 25, 2023, 1:15 p.m....our pipeline. In it, they break down the unique challenges of our platform and how we were able to address...
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  2022 Depositor WeekTypeBlog PostPublishedDec. 12, 2022, 2:15 p.m....on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame Thursday: 8 Great Depositor Research Stories...
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  #Deckthelab Contest Winners 2017TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m....used tip boxes for a lab supply tree and set a challenge for her lab mates to create the best-recycled ...
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  2021 Deck the Lab Winners!TypeBlog PostPublishedJan. 25, 2022, 2:15 p.m.... Though 2021 had more than its fair share of challenges, the annual Deck the Lab Contest brought us smiles...
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  Podcast: A Malawian Professor's Path to Biotech ResearchTypeBlog PostPublishedMay 24, 2016, 2:30 p.m....Kingdom's PhD projects - HIV inhibitors, biofuels, challenges in Malawi, and more 23:45-28:07 : How Seeding...
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  Deck the Lab 2022!TypeBlog PostPublishedJan. 17, 2023, 2:15 p.m....used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint. #deckthelab...
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  Interview with Bart Newland, Addgene’s Newest Board MemberTypeBlog PostPublishedAug. 3, 2021, 1:15 p.m....travel and that's been difficult this past year and a half. My wife and I went to Patagonia in February of ...