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We narrowed to 463 results for: MEA

Showing: 161 - 180 of 463 results

  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving skills” and...because I can resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing ...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...

  3. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...designing a website users could easily navigate. This meant we could grow accessibility alongside our repository...beginners” on r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process...

  4. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...put scientific inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents...changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well in a 96...

  5. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...protein of interest. The tag was developed as a means to purify a protein based on the affinity of the...losing your protein of interest. But that doesn’t mean longer is always better - the longer the tag, the...

  6. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...

  8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...each part of a split fluorescent protein (FP) and measure the signal produced when the candidate proteins...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...

  9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...particles (VLPs). VLPs lack viral genomes which means they are non-infectious and non-replicative. Nanoblades...iPSCs and BM cells after Nanoblade treatment as measured by their maintenance of pluripotency marker expression...

  10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...strains share a common genetic background, which means that each mouse is genetically identical and all...maximum genetic heterozygosity in the population, meaning they more closely mimic the genetic diversity of...

  11. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle... (or BSL-2+) lab and require additional safety measures.   General viral handling safety   Containment...

  12. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...this does not mean that you should apply for everything that’s out there, it just means that you shouldn...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the inverse...Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...do it well" – even the smallest program. I don't mean have fancy food or a fantastic view (these are nice...Timing is everything Many of my suggestions are meant to keep the event on time. Nobody likes a program...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ... control assays for AAVs. These steps include measuring viral titer and assessing purity. But Addgene ...get hits to “random” genes. Do these hits always mean the sample is contaminated? No - what really matters...

  17. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... sum game - that is, if I get what I want, that means you don’t. In this mindset, one of us wins, the ... negotiation is the most straightforward, it can mean that everyone leaves disappointed (we’ve all won...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et...found a way to use the accumulation of indels to measure time. These plasmids with different promoters driving...

  19. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...limit can feel like a lot of pressure. However, it means that your manager (and you) will be motivated to...time, you’ll know more people in industry, which means you’re more likely to have the right contacts and...

  20. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...have died, usually after four to five days. This means that viral production takes a little bit longer ...Titers: Where Do They Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production...
Showing: 161 - 180 of 463 results