We narrowed to 465 results for: MEA

...designing a website users could easily navigate. This meant we could grow accessibility alongside our repository...beginners” on r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process...

... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...

...lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address this... E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used in vivo to study...

...put scientific inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents...changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well in a 96...

...protein of interest. The tag was developed as a means to purify a protein based on the affinity of the...losing your protein of interest. But that doesn’t mean longer is always better - the longer the tag, the...

...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...

...each part of a split fluorescent protein (FP) and measure the signal produced when the candidate proteins...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...

...particles (VLPs). VLPs lack viral genomes which means they are non-infectious and non-replicative. Nanoblades...iPSCs and BM cells after Nanoblade treatment as measured by their maintenance of pluripotency marker expression...

...strains share a common genetic background, which means that each mouse is genetically identical and all...maximum genetic heterozygosity in the population, meaning they more closely mimic the genetic diversity of...

...genomes/mL) can be measured by: Optical density: particle concentration is measured by looking at absorbance...

... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle... (or BSL-2+) lab and require additional safety measures.   General viral handling safety   Containment...

...this does not mean that you should apply for everything that’s out there, it just means that you shouldn...

...FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the inverse...Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn...

...do it well" – even the smallest program. I don't mean have fancy food or a fantastic view (these are nice...Timing is everything Many of my suggestions are meant to keep the event on time. Nobody likes a program...

... sum game - that is, if I get what I want, that means you don’t. In this mindset, one of us wins, the ... negotiation is the most straightforward, it can mean that everyone leaves disappointed (we’ve all won...

...Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et...found a way to use the accumulation of indels to measure time. These plasmids with different promoters driving...

...cell needs to be fixed and the cell membrane permeabilized in order for the antibodies to reach their intracellular...colocalization experiments in a scientific and meaningful way it is important to choose the appropriate...

...have died, usually after four to five days. This means that viral production takes a little bit longer ...Titers: Where Do They Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production...

...aim for somewhere between 30x and 200x coverage, meaning that you need 30 to 200 times as many colonies ...recombination, we recommend ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura...

...limit can feel like a lot of pressure. However, it means that your manager (and you) will be motivated to...time, you’ll know more people in industry, which means you’re more likely to have the right contacts and...