Skip to main content
Addgene
Showing: 161 - 180 of 400 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...location of the ribosome, TRICK (Translating RNA Imaging by Coat protein Knock-off) instead takes advantage... M., et al. “Translation. An RNA biosensor for imaging the first round of translation from single cells...

  2. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...brightest resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons)....maintaining high peak fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled...

  3. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ... more have been critical for pandemic research. Image from Reproducibility for Everyone. Case study...Comparative tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications...

  4. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...bacteria will be stained red or pink, as shown in the image below. An example of Gram positive (purple...purple) and Gram negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio...

  6. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F,...small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb...

  8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve introduced...fluorescence.  Figure 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...    Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay...actually celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing...different antibodies produced by the hybridoma. Image from Bradbury et al., 2018.   Another barrier...

  11. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...of them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...

  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 ...desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682/tutorials...

  13. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... space that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher...often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two ...

  14. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... molecules are clearly associated with cellular damage and dysfunction, it is unclear what the precise... that is now vulnerable to various forms of DNA damage, and these structures can in turn lead to genome...

  16. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Cell Collection at Coriell. More information and images generated from these cell lines can be found in...fluorescent proteins as described here enables live cell imaging to study cellular structures and processes and ...

  19. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep 20;111(6):1119-31...2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...
Showing: 161 - 180 of 400 results