We narrowed to 473 results for: NOL
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TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m....perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...
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Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m.... Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on Addgene.org... Hot Plasmid Pages to See the Latest Plasmid Technologies ... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....knockout of genes in the immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590...subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m.... curator of the thousands of amazing plasmid technologies contributed by our depositors, Addgene strives...certainly not least, we often feature new plasmid technologies on our blog and encourage you to fill out this... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....reported doing in a paper that appeared in Nature Biotechnology back in 2011 and then in another Nature Methods...protein for in vivo imaging. Filonov et al (Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.) Near-infrared... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....intertwined. In this blog we will review how CRISPR technology has brought drug and genetic screens together...of control have also been added to the CRISPR technology to allow time-controlled edits to occur, such... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....rapidly generate stable transgenic cell lines. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. PubMed... modify clinical-grade T cells to target CD19. Immunol Rev. 2014 Jan;257(1):181-90. doi: 10.1111/imr.12137... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled "Advances...amazement at how much has been done with CRISPR technology in just 2 years and excitement about how much... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....more standardized methods for evaluating CRISPR technologies, including the ever important on/off-target ... are aimed at improving translation of CRISPR technology, but they would also bring benefits to researchers... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m.... Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m.... development, vaccine development, and assay technologies makes her a perfect fit for Addgene. She was...experience, soon directed himself to finding “immunological and virological solutions to crises like those... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....at all levels. Jargon is field-specific terminology or language. This can refer both to terms that...that are only used in the field and terminology that is widely used but has a very specific use-case (definition... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative. Our first collection ...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....female. HEKs are generally cultured as an adherent monolayer, though derivatives have been adapted for growth...Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389/fbioe.2021.796991... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....our network. We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m.... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen biology to treat...