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Showing: 161 - 180 of 368 results

  2. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...hydroxybenzylidene imidazolinone (DFHBI) with relatively low fluorescence in vivo, the Jaffrey lab used...

  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...construct [1,2], i.e. positioning of the FP sequence relative to cell compartment targeting sequences, which...to characterize FP-fusion proteins functionally relative to the untagged protein of interest. To avoid ...

  5. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...multiplexing is that you can ask how multiple proteins relate to one another in the same sample. For this strategy... The biggest difference between IF and ICC/IHC relates to the type of secondary antibody you use, so as...

  6. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...scFvs are also used for eye diseases, such as age-related macular degeneration (AMD) since direction injection...cell. When a CAR has an scFv that binds cancer-related antigens, binding of the scFv part of the receptor...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists...

  8. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...provided as an explanation for why they are paid relatively low wages (Stephan estimates that postdocs are...that PIs no longer save grant money by paying relatively low postdoc salaries as opposed to Staff Scientists...

  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...

  10. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene...

  11. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...left me optimistic that I would find a genetics related role soon after finishing my MSc. The masters program...I had received all the training and experience relating to good manufacturing practice (GMP) and good ...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ... controls how many false positives are allowed relative to true positives. Both metrics can be used to...and plot your data in ascending numerical value relative to quantiles derived from a theoretical distribution...

  14. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    .... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...AAV selection, there are three important factors related to location. Location (1): What tissue? Location...

  15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...high Cas9 MOI considering that A549 cells are relatively easy to transduce. I ended up with too many Cas9...on genomic DNA. The surveyor assay itself was relatively easy. Figure 4: Detection of edited BRAF...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...your own favorite Reference Library, for example related to strategies beyond SpCas9, or species not currently... other species, and libraries using strategies related to other nucleases as well as inhibitory and activating...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail.... The advantages of this method are that it’s relatively simple to implement and doesn’t require time-...

  19. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...rather astonishing ability to...well, annoy. Fly-related Issues in the Lab A significant drawback of working...
Showing: 161 - 180 of 368 results