Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 854 results for: REN

Showing: 161 - 180 of 854 results

  1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...leverages the strengths of PRIDICT/PRIDICT2.0 while accounting for some of the inherent inaccuracies of...focus on gene editing techniques. References and resources References Akhtar, W., De Jong, J., Pindyurin...means we could design hundreds to thousands of different pegRNAs for each desired edit, it simply becomes...many more. Once we had our datasets with all the different features and editing efficiencies, we could train...offline via GitHub. The model provides users with different designs of pegRNAs, ranked by their PRIDICT/PRIDICT2.0... predictive power is sometimes limited across different targets due to variations in chromatin context... Our recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024...

  2. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...yours does and then use that time! Different platforms have different quirks, so remember to practice on...that requires its students to do rotations in different labs before selecting an advisor, it is always...points and keeping them at-hand, you can quickly reference your questions about the PI’s research or the ...the name of the award you won for your conference presentation. It can help soothe your nerves and make ...promoted during COVID-19, online meetings with current students, faculty, and staff have become a new ...of just the program requirements: by talking to current students about the work environment and social ...using the virtual world to your advantage can strengthen your application and help you decide if a program...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria.... Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined...plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi integrates downstream...following IPTG induction of conjugation genes and bi-parental mating. Following excision from the B. subtilis...a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain a chromosomal copy...components is transferred to recipient bacteria by bi-parental mating. The ICE integrates into the trnS-leu2 ... of guides, promoters, selection markers, and different versions of dCas9. Following integration, Mobile-CRISPRi...

  4. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...overlapping markers. Compensation/reference control(s) Compensation or “reference” controls are samples stained...spectral flow cytometry are more alike than they are different, there are a few aspects in which spectral flow...complex mathematical algorithm and single stained reference controls to distinguish one fluorophore from another...   Figure 3: Similarity index across 12 different colors on a three laser spectral flow cytometer...conventional or spectral flow. In spectral flow, reference controls tell the software to look for that exact...is not the same as your single color control. Reference controls: Beads or cells? Compensation beads are...new panel on a spectral cytometer, you prepare reference controls of both beads and cells for a single ...

  5. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...Organizing a science conference for the public How does one organize a public scientific conference? As a member...first-time event called DayCon. DayCon is a one-day conference aimed at the general public that provides an ... commitment of SITN members. SITN and DayCon Currently in its 16th year, Science in the News has two ...scientists and the public and help make science more transparent. Second, SITN helps graduate students develop...graduate student at Harvard, who wanted to provide a conference-style experience that was accessible to the general...picked a theme (Bio-Medley) to showcase SITN’s strength in the biomedical sciences and decided to offer... make DayCon a yearly event and to expand the conference to accommodate a larger number of attendees. ...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... of antibiotics, but the field is currently undergoing a renaissance. Yosef et al. designed a two-phage...lysogenic phage, rendering the population sensitive to antibiotics. Created with BioRender.com. Phages ...swift, as few as five years after introduction. Current antibiotic strategies are nonspecific - they harm...defense against bacteria. Created with BioRender.com.   The problem of antibiotic resistance ...alter an individual’s native, beneficial flora. Currently, there is no antibiotic strategy available to ...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

  7. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...the host. The cause, manifestation, diagnosis, recurrence, and treatment of cancer have been extensively...compromised either due to age, infections, or other strenuous biological changes. In contrast to the acute immune... cytokines like TGFβ and IL-6. However, when strengthened through efforts to boost the activity of the... most commonly used immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies .... Sipuleucel-T by the Dendreon Corporation is currently the only FDA-approved DC therapy for prostate ...only just touched the surface of immunotherapies currently under study. Check out this review for an in-depth...manifestation to their specific interactions with different cell types. A thorough understanding of the genotypic...

  8. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...host species. Created with BioRender.com. How are shuttle vectors different from other plasmids? If ...protects against any of them. But different aminoglycosides work on different host species: kanamycin targets... unrelated host species, even from completely different kingdoms of life. That’s convenient for researchers... Shuttle vectors have to address several big differences in the ways vectors are used across species: ...resistance gene on your vector might require two different selection molecules! It all depends on how the...Auxotrophic selection Another option is to use a different method of selection entirely. Auxotrophic selection...host range,” these terms are typically used in different (though overlapping) ways. Broad host range plasmids...

  9. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ... can separate the DNA strands. Created with BioRender.com. Once DnaA breaks the local hydrogen bonds...Prokaryotic chromosomal replication. Adapted from BioRender template. Since DnaA binding is necessary ...proteins also contribute to this regulation in different ways: methylating newly synthesized DNA, blocking...numbers can allow RepA to link iterons from two different plasmids, forming a pair of “handcuffs” that blocks...RepA proteins form complexes between iterons on different plasmids in a process called "handcuffing," which...folding, blocking replication. Created with BioRender.com. Plasmids under stringent control of replication...was updated by Emily P. Bentley in June 2024.  References and resources Further Reading Quiñones-Valles...

  10. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...soils. Dendrobatidae (Lauren O’Connell)  Poison frogs are great for studying parenting strategies and make...land. Current tools are all for Xenopus (aquatic frogs), but there are few genetic tools currently for ...Genomic Tools) Program and I met scientists from 21 different labs who clearly also share this wonder of the...convergent evolution because electric organs arose in different branches of the evolutionary tree at least 6-7...editing in the brain allows the squid to create a different protein. Did you know that most cephalopods die...behavior. For example, two similar species have very different social behavior. One is monogamous and one is ...new tools being developed and shared for many different organisms. Do you have a favorite organism you...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...virus:  Lowering cytotoxicity. Different envelope proteins have different levels of cytotoxicity and the...Altering sensitivity to serum. Different pseudotyped viruses are differentially inactivated by nonspecific...Table 1 (Cronin et al., 2005)     References and Resources References: Crawford KHD, Eguia R, Dingens AS...protocol for generating lentivirus, but you use a different envelope glycoprotein instead of the wild-type...review articles to find tables and information on different viral membranes, tropism, and advantages and disadvantages....084 Li Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for ...

  12. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...for other antibody topics.     References and resources References:  Björck L, Kronvall G (1984) Purification...elute and measure the target. However, the two different approaches allow for optimized strategies for ...amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein extracts... appropriate for most situations.  A slightly different method is desirable if the antibody is expected...Protein G have an affinity for immunoglobulins, the strength of the interaction varies between antibody host...than those of agarose beads, despite the size difference. Magnetic beads are particularly advantageous...many factors, including salt concentration, ionic strength and pH, affect binding the lysis buffer may need...

  13. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...expect, different companies produce different products and therefore need people with different areas of...: vast practical lab expertise, experience in different scientific fields, and knowledge of how to troubleshoot... as: “Can I use this product under conditions different from those specified on its data sheet?” or “Do... questions customers ask, and there are also differences in customers’ personalities and their expectations...do jobs in technical support have? There are different names for technical support roles. Examples are...distinction between these job titles, they may be different regarding the levels of lab experience and scientific...communication skills by working with people from many different backgrounds and realizing that the same approach...

  14. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...going to cite hundreds of references) and it’s better to keep your references organized from the beginning...the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this post...criteria to shape the document. Get and use a reference management program (e.g., EndNote, Papers, Mendeley...Mendeley, etc.) You’re going to be managing a lot of references. I cite as I write, meaning I use the software...in real time as I wrote, so each statement was referenced even in the roughest version of the draft and...that people can go back and read the original reference for the data. For example, we summarized how frequently...place to get a sense of the similarities and differences among proteins in the class. Offer your perspective...

  15. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...educational training, and conferences, and synthesizing this knowledge into a coherent narrative that gives...best with diverse teams, to leverage the preferences of different personality types, and to devise strategies...biotech clients. In this realm, there are a few different kinds of challenges that consultants get called...companies. Medical Affairs groups work on building awareness among physicians and scientists about clinical...consulting firms coach and counsel employees on the different personality and work styles (e.g., Myers-Briggs...prepare you for a successful career in several different fields. While some people continue to enjoy the...BioCentury can be an engaging way to learn about the trends and workings of the healthcare industry. Finally...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...doping to forensics, DNA sequencing to drug testing. You can find Nick on LinkedIn. References 1. Skloot...Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification ...the cell lines deposited with them and used by different researchers are free of contamination and match...from this bank. I am often asked, “What is the difference between materials available from commercial repositories...the same cell line from a colleague?”. The main difference is the QC and validation carried out by the commercial... The same test is so good that it is used as a forensic technique (aka DNA fingerprinting) that can place... produce a new batch once you’ve passaged your current batch too many times or you suspect there is contamination...

  17. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...are communicating across disciplines or doing different tasks. But these challenges can be exaggerated...equal opportunity to contribute. Using video conferencing tools like Zoom or Google Hangouts is a great...great way to include researchers from different locations. Having virtual face-to-face meetings as opposed... collaboration includes scientists working in different time zones, it’s important that everyone can access...project at the International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances...techniques are being performed the same way at different sites. I did a lab exchange as part of the collaboration...allowed me to investigate my protein of interest in a French clinical cohort, neither of which I would have ...

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    .... The plasmids within it contain a variety of different promoters followed by leader sequences (encoding... at the BIOFAB showed that expression of many different genes was predictable based solely on the promoters...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...or protease sites to remove the epitope tags. Different plasmids in the kit can be used to purify proteins...prokaryotic expression in recent years, with many different systems now available. However, it can be difficult...ORF clones to allow testing and comparison of different cell-free expression systems; the Cell-free expression...bind specific RNA bases. By swapping out these different modular repeat segments, you can create a PUF ...

  19. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...what we need to know in order to even assess the current state of research became more clear throughout ...that, even though postdoc associations across different universities are trying to compile more comprehensive...path,” but individual institutions can have many different names for the positions commonly thought of as...the Starting Salary for Postdocs to $50,000 (the current median postdoc starting salary as reported by Paula...Student Career Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. ...achieved. Of note, it was highlighted during the conference that just increasing funding for academic research... At Addgene we try to do our part in boosting awareness of various career opportunities through our career...

  20. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...Why the HEK not? References and Resources References Shaw G., et al., Preferential transformation of ...determined it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney...were isolated from is still a mystery, but it’s currently assumed that the cells came from an aborted fetus...genetically female. HEKs are generally cultured as an adherent monolayer, though derivatives have been adapted...have some neuronal qualities.) While HEKs probably aren’t a great model of kidney cell biology, there are...
Showing: 161 - 180 of 854 results