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We narrowed to 599 results for: REV;

Showing: 161 - 180 of 599 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Utility. Annual Review of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-...Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to receptors...

  2. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...you’re ready. Plus, what better addition to peer review than having your plasmids sequenced and openly ...moved on to their next position. We do routinely review deposits that are left in the hold status and reach...depositing plasmids with Addgene! We'll continue to review our policies to make sure they meet our goals, ...

  3. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ... Need a quick review of the different immune responses? We got you! You can also review antibodies in ...Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector...

  4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...sequence reverse complement tool: Enter a DNA sequence and the program will give you the reverse sequence...sequence, complement sequence, or reverse and complement sequence.  Filter DNA tool: Removes non-DNA characters...

  5. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...analyses with the relevant figures from a literature review. No more hunting through a whole folder of papers...might include a confirmatory image to show that a previously used reagent performs well, or you might publish... one such research product which can convince reviewers of your expertise and insight. We recommend sharing...

  6. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... a single point mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3)..... Addgene has empowered researchers to harness previous experimental successes and further develop the... FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature Reviews Microbiology. 2011 Jun; 9, 467-477. Bondy-Denomy...

  7. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...standard AAV QC regimen does not address this.  Previous studies have demonstrated that NGS is an effective...tagmentation preparation approaches described previously require a preliminary double stranding step. ...specific signature sequences in the data. Unlike previously described thermodynamics-based methods of serotype...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...species and applications. In this blog, we will review the most common tags, the antibodies used to detect...optimizations and specific monoclonal antibodies. Below we review the most commonly available tags and their antibodies...Myc was developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit...

  9. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments...to do is co-transform pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter...confined experiments as well as those requiring reversibility that you cannot do with AraC,” says Di Ventura...

  10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily transferrable...oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and... of this to control a metabolic network in S. cerevisiae by using three RNA programs to differentially...

  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...with the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled...problem was I knew I had a knowledge gap. I had no previous business, marketing or sales training and knew...

  12. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned...fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP...PMCID: PMC3790201. Elmore, Susan. "Apoptosis: a review of programmed cell death." Toxicologic pathology...

  13. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...introductions to potential publications or prepare a review article. These are both options that can be done... started early on introductions and literature reviews could make the months leading up to your defense...Consider submitting a registered report, where peer review is based on the methods, not the results. Also ...

  14. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ... is conserved in all living organisms and has previously been used for multiplex Cas9-nuclease editing...existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream lengthy..._VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP...

  15. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Using this road map, and what was known about previously characterized TFs, the Zhang group were able ...most immediately useful for TF library screens. Previously engineered TF libraries were not as comprehensive...transformation and amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications...

  16. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...scientists Anyone can become a member of BosLab and previous laboratory experience is not required. Our members...microbiome project. This project was inspired by previous city-wide genomic studies and our study will involve...

  17. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...educational and great for lab life. These apps prevent you from constantly searching the interwebs for...important biological information, amino acid abbreviations, and structures into one convenient resource...say that I still have the single and triple abbreviations of the amino acids memorized, but the structures...

  19. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...strategy that works. But, those darn cancers have revealed many tricks up their sleeves and most patients...experiments. But, there have been two huge challenges preventing us from testing this hypothesis: There was no... this publication We also wrote an extensive review on the exciting new field we call “precision functional...
Showing: 161 - 180 of 599 results