We narrowed to 251 results for: SPI;

1. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ...in Germany. I attended this symposium seeking inspiration to resolve the technical hurtle of monitoring...

2. To Each HIS Own

   Type

   Blog Post

   Published

   March 2, 2023, 2:15 p.m.

   ... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins...

3. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...Python or MATLAB. Choosing Python makes it easy to spin up a read-only Jupyter notebook for them and let...

4. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is also suitable for optogenetic ...

5. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...growing dialogue on the state of a system that, despite all of its successes, has many deep-rooted flaws...

6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...Check out the hashtag #365papers on Twitter for inspiration and accountability. There are a few different...

7. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   Published

   Sept. 27, 2017, 1:23 p.m.

   ... careers are in advance of yours, but also get inspired and meet peers. Join societies like National Science...

8. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...measure the tension in the helical structure of spider silk, or the activity of a protein in a synapse...

9. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...ultracentrifugation, centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation...

10. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...things your lab can do to get into the robotics spirit include: Start using electronic multichannel and...

11. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...with a penicillin resistance plasmid can grow in spite of a hostile Penicillium fungus trying to kill them...

12. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...currently working on giving this treatment a CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma,...

13. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ...for students interested in leading the process despite the recent advance. Similarly, grad students throughout...

14. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

15. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy...

16. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...see how low we could go. We prepared a range of spiked samples from 0.1-20% and discovered that, with ...

17. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...dish containing the circled clones is removed by aspiration and the dish left covered (to avoid drying out...

18. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized...

19. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...

20. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A., Sousa, A. A., Tsai, S. Q., ...