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TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m....targeting the mouse and human kinomes. While any subset of the genome is limited by definition, the kinome...plasmids contain the same 3-4 gRNAs found in the first subset of the pooled library. The individual plasmids ...
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Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....a few species of bacteria and sometimes, only a subset of strains of the same bacterial species are affected...developments at Phage Directory and in the phage field, subscribe to their newsletter, Capsid and Tail. Additional... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....once science was only seen as a serious academic subject, it is now being appreciated on an aesthetic level...which could promote conservation efforts, as the subject matter is seen to have increased value and is appreciated... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....locus may be useful to study haploinsufficiency. Subsequent modifications at the remaining allele within ... Lin C, et al. An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....expectations were for the intended position. After submitting my application, I was contacted to participate...discrepancies between what we sequenced in house and the submitted sequences were a concern to the plasmid function... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....or antibodies against common tumor markers to subsequently capture cancerous cells expressing low levels...depositing your plasmids with Addgene. Howarth: We submit all our plasmids that may well be of use to others... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....dopaminergic neurons in a brain region called the substantia nigra, which is the main source of the body’s...sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar) DA affinity and high-magnitude responses (increases... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....serves to stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps... short DNA fragments can then be purified for subsequent library preparation and high-throughput sequencing... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....determine potential water contaminants and to subsequently develop technologies to protect fresh water ...Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for updates and future collaborations... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....prefer to be approached as peers rather than subordinates Need mental space and dislike intrusions (this...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....reasons to feel obligated too, as experts on the subject brought out in a Kavli Foundation Round Table. ... be a good way to follow news about particular subjects or find out what others are reading. Check out... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m.... deposit agreement must be put in place. And subsequently, every time that material is requested, a material...find some easy talking points for broaching this subject with your institution’s tech transfer professionals... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP...choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....to what the research had to say. Moreover, this subject allowed me to engage in findings relevant to my...areas in which I knew very little. Diving into subjects like risk perception, scientific uncertainty and... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....Accelerate Research Discoveries) Award attracted submissions from 99 universities and foundations in 21 countries...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m.... be helping the untagged protein enter cells. Subsequent work found that both NaCl and non-detergent sulfobetaine... be used to transduce Cas9 and a gRNA, which subsequently enter the nucleus and cleave the specified target... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....hairpins into the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize... used to visualize relative locations of the subtelomeric region of the pericentromeric region (PR1), ...