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We narrowed to 370 results for: SUP

Showing: 161 - 180 of 370 results

  2. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...that you ask a PI or mentor (your supervisor/ a previous supervisor / someone else in the department) ...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...of CpG islands in the promoter region of tumor suppressor genes can repress their transcription and inactivate...al. "Change in paternal grandmothers early food supply influenced cardiovascular mortality of the female...

  4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...Mature Nanoblades then bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9...After Gag-Cas9 is incorporated, the MLV protease, supplied as part of the MLV Gag-Pol polyprotein, cleaves...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...polyclonal antibodies, creating a potentially unlimited supply of identical antibodies (See Fig 1.). rAbs are ...

  6. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... accidental release. Science relies on popular support, which in turn depends on widespread trust in scientists...environment, they must never be used without popular support2. An accidental release could delay real-world ...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...CRISPR screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries...AAV CRISPR screens have been used to map tumor suppressors in mouse liver, interrogate immune cells to improve...

  8. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...plasmids/year from 2017 to 2023. This growth is supported by new labs joining the Addgene CRISPR community...Barrangou for his authorial contributions to, and support for, this and previous Addgene CRISPR publications...

  9. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...AstraZeneca, scientists who get postdocs and do not support or manage them well are simply no longer allowed...the opportunity to do some great science in a supportive environment. Both paths will give you myriad ...

  11. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...which includes primers and a ddPCR supermix. Note that the supermix and droplet oil that you use should...

  12. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... was far from an isolated case. Our scientific support team answers hundreds of AAV questions every year...Figure 2.) At the same time, when scientists who support open science, but do not share their own data -...

  13. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...it just as much in my current role as Customer Support and Operations Manager at Addgene. In this role...The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene and is interested...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

  15. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... to eliminate the use of antibiotics by their suppliers, and public health and medical organizations are...win back ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...vectors not only available, but accessible. The supporting data and community engagement required to do ...lessons learned from this process have shaped and supported our growth and development. We’ve shared what ...

  17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...variety of cellular processes and an antitoxin that suppresses the toxin’s activity. The key to these systems...long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA antitoxin...

  18. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...be resistant to change. Ask your peers or your superiors to explain their rationale when they’re resistant... currently plaguing the scientific community is supply chain shortages. When flasks, conical tubes or ...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...genomic site of interest. The donor template is supplied separately. To use this system, you’d first introduce...with the original system, the pEcCas/pEcgRNA is superior in several ways: The pEcCas plasmid can be operated...
Showing: 161 - 180 of 370 results