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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 378 results for: SUP

Showing: 161 - 180 of 378 results

  1. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...plasticware is readily available from scientific suppliers and glassware should be treated with a DEPC solution...material is removed via centrifugation and the supernatant is extracted with chloroform as in the above ...

  2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...these cells may be considered "natural tumor suppressors." A growing interest in the cancer microenvironment...Myoepithelial cells: autocrine and paracrine suppressors of breast cancer progression." Journal of mammary...

  3. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...Some organizations, including some journals, also support open peer review or curation models where editors...Along with other open-science organizations, we support scientists in sharing their results and materials...

  4. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...into a different meaningful career in science. Supervisors in the academic infrastructure are not always...be helpful having a coach that can offer extra support for issues of diversity in science.  A great person...

  5. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...Mature Nanoblades then bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9...After Gag-Cas9 is incorporated, the MLV protease, supplied as part of the MLV Gag-Pol polyprotein, cleaves...

  6. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...polyclonal antibodies, creating a potentially unlimited supply of identical antibodies (See Fig 1.). rAbs are ...

  7. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...that you ask a PI or mentor (your supervisor/ a previous supervisor / someone else in the department) ...

  8. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...of CpG islands in the promoter region of tumor suppressor genes can repress their transcription and inactivate...al. "Change in paternal grandmothers early food supply influenced cardiovascular mortality of the female...

  10. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...for long-term imaging or advanced techniques like super-resolution microscopy. To make this segment of the...depending on the application. Figure 1: Super-resolution imaging of actin filaments with mGold2...

  11. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...CRISPR screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries...AAV CRISPR screens have been used to map tumor suppressors in mouse liver, interrogate immune cells to improve...

  12. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...plasmids/year from 2017 to 2023. This growth is supported by new labs joining the Addgene CRISPR community...Barrangou for his authorial contributions to, and support for, this and previous Addgene CRISPR publications...

  13. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...AstraZeneca, scientists who get postdocs and do not support or manage them well are simply no longer allowed...the opportunity to do some great science in a supportive environment. Both paths will give you myriad ...

  15. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... accidental release. Science relies on popular support, which in turn depends on widespread trust in scientists...environment, they must never be used without popular support2. An accidental release could delay real-world ...

  16. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

  17. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...which includes primers and a ddPCR supermix. Note that the supermix and droplet oil that you use should...

  18. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...it just as much in my current role as Customer Support and Operations Manager at Addgene. In this role...The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene and is interested...

  19. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... was far from an isolated case. Our scientific support team answers hundreds of AAV questions every year...Figure 2.) At the same time, when scientists who support open science, but do not share their own data -...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... to eliminate the use of antibiotics by their suppliers, and public health and medical organizations are...win back ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark...
Showing: 161 - 180 of 378 results