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Showing: 161 - 180 of 426 results

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...tasks. EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...

  4. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro...and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits and...

  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9:...gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...inventory management systems to enable efficient, tracked production and distribution of viral samples. The...

  8. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...components. Transfer cPPT Central polypurine tract; recognition site for proviral DNA synthesis. Increases...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. This post was written...

  10. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion channel proteins, they are not always easy to track at the transcriptional or translational level. However...fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states (Matthew et al., 2018). These...

  11. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... in each plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing its set... gRNA Read How Barcodes Can Be Used in Lineage Tracing Read about CRISPR/Optogentics Tools Resources ...

  12. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...through our deposit process, and then we carefully track samples through our lab using barcoded tubes and...Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View ...

  13. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...replaces the targeted gene and provides a way to track successfully engineered cells. Similar to transgenic... the cell can occur. Similar systems include tetracycline or doxycycline mediated activation of Cre to...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...

  15. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...PubMed user searches, such as clicks to read the abstract or full text of the paper, publication date, a...publications and figures using machine learning to extract important features such as influential citations...

  16. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...

  17. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can ...chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris...

  19. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Pooled libraries are a bit more challenging to...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org...

  20. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...
Showing: 161 - 180 of 426 results