We narrowed to 435 results for: Trac

1. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   Blog Post

   Published

   June 4, 2019, 1:02 p.m.

   ...mCherry ratios. Genomic DNA from each bin are then extracted and sequenced. Chiasson is currently using VAMP-seq...constitutively fluorescent reporter can also be used to track how one population responds to a second population...

2. Plasmids 101: NGS Quality Control for Pooled Libraries

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   Blog Post

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   Oct. 26, 2017, 1:59 p.m.

   ...then scraped off the plates and pelleted for DNA extraction. Prepare library product for next-generation ...next-generation sequencing Once the DNA has been extracted, the representation of the library should be confirmed using...

3. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   Blog Post

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   June 1, 2021, 1:15 p.m.

   ...help researchers working on COVID-19. Its origins trace back to March 2020, when the first coronavirus lockdown...recommendation for the correct statistical test can save a retraction. Today, collaborations are limited by the difficulty...

4. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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   Blog Post

   Published

   Nov. 27, 2018, 2 p.m.

   ...Klebsiella and Pseudomonas isolates causing urinary tract infections. This project aims to examine the antibiotic...research related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology...

5. Resume Writing for Non-academic Science Careers

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   Blog Post

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   Dec. 18, 2014, 4:06 p.m.

   ...of that, perhaps you were involved in some extracurricular activities – did you volunteer to do science..., University of British Columbia. Originally on track for a research career, she transitioned into a communications...

6. Finding and Joining Your Dream Lab

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   Blog Post

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   April 8, 2014, 2:50 p.m.

   ...you’re within a year from the end of your PhD or contract, you should be starting to send out polite emails...can. You know you are in the zone when you lose track of time because you are engrossed in a task. Things...

7. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

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   June 6, 2019, 12:41 p.m.

   ...sometimes require an alternative such as a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed...contains an sgRNA insert site and a reporter for tracing the engineered cells in vivo, the non-immunogenic...

8. A Deep Dive into BioBuilder

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   Blog Post

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   Oct. 11, 2017, 1:44 p.m.

   ...Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro...and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits and...

9. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

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   May 11, 2017, 2:30 p.m.

   ...Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About Cancer Immunotherapies...

10. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

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    May 9, 2017, 2:12 p.m.

    ...CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9:...gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang...

11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

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    Aug. 6, 2024, 1:15 p.m.

    ...components. Transfer cPPT Central polypurine tract; recognition site for proviral DNA synthesis. Increases...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. This post was written...

12. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post

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    Dec. 15, 2017, 2:07 p.m.

    ...Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...

13. Targeted Mutagenesis with EvolvR

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    Blog Post

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    Feb. 21, 2019, 1:15 p.m.

    ...tasks. EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...

14. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post

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    March 23, 2017, 2:30 p.m.

    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...inventory management systems to enable efficient, tracked production and distribution of viral samples. The...

15. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

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    Oct. 29, 2019, 2:52 p.m.

    ...ion channel proteins, they are not always easy to track at the transcriptional or translational level. However...fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states (Matthew et al., 2018). These...

16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

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    March 3, 2020, 2:15 p.m.

    ...optogenetic tool for precision in cell lineage tracing Article contributed by Erin Sanders CreLite, designed... and his colleagues evaluated CreLite for cell tracing using the transgenic zebrafish model, ubi:zebrabow...

17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    Blog Post

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    April 12, 2016, 2:30 p.m.

    ... in each plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing its set... gRNA Read How Barcodes Can Be Used in Lineage Tracing Read about CRISPR/Optogentics Tools Resources ...

18. Addgene's Tips for Plasmid Quality Control

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    Blog Post

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    Jan. 14, 2016, 3:30 p.m.

    ...through our deposit process, and then we carefully track samples through our lab using barcoded tubes and...Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View ...

19. Design Tips for Prime Editing

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    Blog Post

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    Jan. 23, 2025, 2:15 p.m.

    ...near point mutations to create 3-base (or longer) tracts of edited bases. DNA mismatch repair (MMR) is less... protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) can improve...

20. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ... Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...