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...library, and we thought it would be a good time to talk about the amplification and verification processes...

...components, removing inducible promoters that could crosstalk with other pathways. They also reduced system ...

...CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between the two fluorescent proteins. If you must...

...hire visits the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one...

...tell people the best part of my job is getting to talk to so many different people! I communicate with ...

...Result of a blue-white screen. Image from Stefan Walkowski.   Restriction digest Another way to distinguish...

...before Cas lets go Figure 1: David Liu gives a talk at the Cell Symposia: Gene- and Cell-based Therapies...

... Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent repositories...

...” and “I hope it isn’t degraded” when scientists talk about their precious RNA samples. RNA is inherently...

...you complete your work. If you’re giving a public talk, ask a friend who works in a different field to ...

...board on their website specifically so people can talk about GCE. In fact, their first protocol is available...

...games.  If I’m not at game, I’m either out for a walk with our new dog, Daisy, or out on golf course! ...

...across your FSH vs reporter plot. In this post, I’ll walk you through how to interpret the antibody readout...

...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening ...

...you admire.  If you see someone who gives a great talk, ask them for advice on speaking. If you see someone...

...research article, presenting a conference poster or talk, or submitting patent or grant applications; to ...

...someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD protein with...

...than one that has never been used in a publication. Talk with your peers. If someone in your field has used...

...the indirect approach - which means it’s time to talk about secondary antibodies. While a primary antibody...

... So in our last celebratory moment, we wanted to walk through some of our favorite milestones and share...