We narrowed to 235 results for: bon;

1. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ...

2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...

3. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...

4. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...

5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...

6. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...

7. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   .../10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan ...

8. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

9. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 Adjust pH ...

10. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

11. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    Published

    Jan. 9, 2020, 1:45 p.m.

    ...it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 characters!...

12. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...(about 2.7kb total)—is inserted into a plasmid backbone for delivery to the cells. As a precautionary ...

13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ...

14. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

15. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...

16. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but...

17. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems...

18. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...the dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the...

19. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...