...your LinkedIn and your resume and prepare business cards. You might not consider being a student or postdoc...postdoc a “business,” but having business cards is a good way to share your information quickly. During the...meet ten new people each day. Exchange business cards. When you’re done networking for the day (or whenever...have a small break), write down some notes on the card so you remember who they are and what you’ve talked...

...ICEBs1) system. ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes, like Mobile-CRISPRi...from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined suite of tools for ...

... homologous recombination events: The plasmid carrying the desired genetic modification integrates into...ShadowR is used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is ... a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for... New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial Genomes. Applied...

...how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered... vivo, in vitro, ex vivo CRISPR screens can be carried out using a few different methods — in vivo, in...outside a host cell’s genome. In this state, AAV cargo can be stably expressed in non-dividing cells for...

...While CRISPR is certainly not the first means to carry out so-called “forward genetic screening experiments...are your cells derived from? Currently, Addgene carries CRISPR libraries that target mouse, human, fly,...genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout, activation,...or a specific class of genes? Addgene currently carries several genome-wide CRISPR libraries and a selection...

...school district that has a lot of parents in STEM careers, chances are they’re wondering how they could contribute... led up to my job at Addgene, which is likely a career path none of them had imagined for a scientist....talked about CRISPR’s molecular mechanism, I was careful to keep it relatively simple. I honed the complexity...testing your SciComm skills or are interested in a career in education. Maybe you just like to see the look...

...non-dividing cells, lentiviral vectors can carry much more genetic cargo (8 kb vs 4.5 kb), and they remain popular..., and transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package ...Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...

..., each package is opened and the contents are carefully inspected to verify that the samples arrived intact...dedicated and detail-oriented lab team that works carefully to catch mistakes. Each sample that is transformed...shared with multiple people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours... time searching for samples and keeps us from discarding tubes in error. The Addgene lab works diligently...

...scheme very nice at first sight in that Addgene takes care of everything including MTA and shipping. Addgene... scientific societies. Addgene: Throughout your career, you have held research appointments in the US ...different parts of the world in my early research career. I've found that research institutes in different... Caroline LaManna...

...Read all of our career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch...engaging your audience. You need to make your audience care, to want to know the answer to your research problem...Watch our career videos ...

...and Sota Hiraga found that if a mini-F plasmid carried a specific 700 bp segment of DNA, the plasmid was... without plasmid addiction systems. For example, Cara Morin and James Kaper used the hok/sok system to...be used over prolonged periods. Using a plasmid carrying the hok/sok toxin-antitoxin system, they were ...223.1 (1992): 41-54. PubMed PMID: 1370544. Morin, Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing...

...students, rotation students, joining a new lab at any career stage, learning a new technique while visiting ...rightfully so! They can provide you with high-level career advice, open doors for you, and teach you valuable...identify a ‘scientific mentor’: someone who provides career advice and offers direction on your scientific ...hasn’t sent you papers or protocols yet, don’t be scared to ask for some! You can email or ask in-person...

...platform to deliver various cargo to a specific DNA locus by directly fusing the cargo to dCas9.  This technique...next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene.  Validated...CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that have...

...Non-Academic Career Decisions Check Out Our Management for Scientists Series Science Career Options Resources...Addgene.org Learn more About Careers at Addgene ... academic career...

..., if you know what you need. Most often, early career scientists aren’t trained to have these types of... and not on the financial aspects. Money often carries an emotional weight. Therefore, too much focus ... a pathology microtome/cryostat, then making a careful decision on the condition and availability of the...XpDesignsMGNT        Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental...

...You've worked hard designing your plasmid – you carefully selected the optimal promoter for your gene of...recognize your little prokaryotic minions that carried out the labor-intensive process of replicating ...is appropriate for propogating your plasmid or carrying out your experiment. History of E. coli strains...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...right homology arm to the desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags ...Yang, Hui, et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated... robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA...

...2007): 1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1–9 mediated...make AAVs useful for a wide variety of experiments, care still needs to be taken when selecting which particular...22418061. PubMed Central PMCID: PMC3381869. 7. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski...

...laboratory. The risk of generating RCL is low but extra care has to be taken especially if you are working with...of interest.   Advantages Disadvantages Can carry large transgenes (>8 kb) Potential for generation...feature for your experiment; it must be chosen carefully. Often researchers want a simple set-up where ...oncogenes for instance should be manipulated with care. See the NIH’s biosafety guidelines for more information...

...and we both won accolades that would launch our careers in science. Fast forward nearly 20 years and ... single author? I’ve seen so few in my 20+ year career that I can’t help but feel a bit dubious when I...Resources on Addgene.org Check out Career's at Addgene ... career...