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We narrowed to 250 results for: cas9 genes

Showing: 161 - 180 of 250 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... applications Gene expression control Researchers are always wanting to manipulate gene expression – turn... Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations in genes of interest, ...viral vector for your gene of interest – an shRNA sequence specific to your gene’s mRNA or a sgRNA targeting... Connie Cepko Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome ...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen...from basic science research all the way to clinical gene therapy. But how can you use these amazing tools... knock it out, etc. It’s how we understand what genes do! Viruses have several options to allow you to...

  2. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology (2014... tools yet. As a genome editing tool, the CRISPR/Cas9 technology has been surrounded by a whirlwind of... parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature biotechnology(2014). PubMed PMID... et al. "DNA targeting specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827...to select a tool based on your project and not general opinion. I could spend a lot of time running through... Online tools dominate the software space but generally rely on less powerful algorithms to detect off-targets...tools available mostly work on any computer but generally require working knowledge of the command line ...

  3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...bacterium expressing dCas9 A plasmid in the host bacterium that expresses gene III. Gene III is under the ...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a...compatible with many Cas domains beyond SpCas9. In order to expand the gene-editing applications and targeting... the expression of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria...activity. A plasmid-based genetic circuit in the bacteria links the expression of gene III to base editor activity... 1: David Liu gives a talk at the Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and...the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to the DNA to induce...

  4. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert genes in a cleavage free manner. The dCas9-SSAP...cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et al, 2022.  Find dCas9-SSAP...of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system ...in comparison to the 62-90% error rate seen with Cas9 editors. The lab believes that this system could...reduces the need to generate “single-use” transgenic lines. Instead, researchers can generate and maintain transgenic...Cre-mediated AAV Targeted Evolution method (M-CREATE) dCas9-SSAP with reduced error rate for long sequence knock-ins...each protein of interest and, for in vivo work, generate a dedicated transgenic line. Using zebrafish as...

  5. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ... will focus on generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next...social media. #AddgeneCRISPRweek on Twitter From May 13 (today!) to May 17, the Addgene Twitter feed will...CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not...

  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...Repair is Used to Edit Genes Read How Non-Homologous End Joining is Used to Delete Genes Additional Resources...long period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target... CRISPR gene therapy, it’s important to realize that DMD is a simpler case than other genetic diseases...there is still no good treatment. The dystrophin gene is very large (79 exons), but much of the sequence...Dystrophin’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on... cells expressed functional dystrophin. Editing genes in vivo is of course much more difficult than in...cells, where precision editing of the dystrophin gene would take place, with minimal risk of off-target...

  7. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...to me: a Cas9 nickase 101 blog post.  On the twelfth day of CRISPR, Addgene gave to me: an updated CRISPR...CRISPR, Addgene gave to me: a primer on prime editing.  On the eleventh day of CRISPR, Addgene gave to...all of them here.  On the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian... mammalian use. On the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing...editing efficiency. On the third day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation ... Delivery (SEND). On the fourth day of CRISPR, Addgene gave to me: enhanced prime editing systems through...manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me: efficient CRISPR editing with a hypercompact...

  8. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...dominant disease-causing gene, or that carry only one copy of a recessive gene. Ma et al. claim that CRISPR...Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse All CRISPR Plasmids... samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated them...genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all cases, they did not observe...authors to title the paper “Correction of a pathogenic gene mutation..” rather than using term ‘editing.’ While...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...Reference Ma, Hong, et al. "Correction of a pathogenic gene mutation in human embryos." Nature. Epub 02 August...

  9. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...scarless cloning. The cloning scheme is as follows: the gene of interest is designed with Type IIS sites (such... allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy...recognition site(s) thus eliminating these from your gene of interest. PCR products are then digested with...ligated following a heat inactivation step. If your genes of interest or destination vector contain multiple...are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in ...More Resources at Addgene:  Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning...

  10. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ..."Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja (2017...required for the onset of gene expression. However, it has yet to be determined what gene expression looks like...exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient and non-pathogenic...Additional Resources on the Addgene Blog AAV: A Versatile Viral Tool for Gene Expression in Mammals A Match...Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors...transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport is...Otherwise, using a specific system for controlling gene expression such as Cre-lox, may be sufficient to...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...can also make accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to...be useful to our model  so that we can use CRISPR/cas9 to build the mutants, analyse their motor behaviour...especially when trying to explore the links between genetics and anatomy, which are not yet fully understood...many different mutants because the Caenorhabditis Genetics Center (CGC) at the University of Minnesota has...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...

  12. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...eleventh day of CRISPR, Addgene gave to me: methods for adenoviral delivery of CRISPR/Cas9.  On the twelfth ... CRISPR, Addgene gave to me: a primer on prime editing.  On the second day of CRISPR, Addgene gave to ...On the fourth day of CRISPR, Addgene gave to me: ways to use optogenetics to control CRISPR editing.  ... day of CRISPR, Addgene gave to me: updates on base editing from the first generation of base editors ...the eighth generation (and dual base editors, too!).  On the seventh day of CRISPR, Addgene gave to me... of CRISPR, Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to me:...🎶 On the 12 days of CRISPR, Addgene gave to me… 🎶 A few years ago we used the #12DaysOfCRIPSR hashtag...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... insert the FP gene into the target gene’s native locus, but introducing large genetic changes in C. elegans...the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease that has...glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at Addgene CasRx ...cassette exons and disrupts genes with alternative exons. Get the library from Addgene New from the viral...library inhibits over 18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed...So how can you use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet... validated this tool by targeting six different genes whose protein products have distinct localizations...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...control expression of their genes of interest. While recombinases are powerful gene expression tools in and...targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library here A new SaCas9 ABE variant...available from Addgene, head over to our CRISPR Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent...blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases are enzymes... ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact that ...precisely controlled through the use of optogenetics. Optogenetic tools use light to induce conformational...Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting...

  15. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...depending on above selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism...for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed for the Fragmid...plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating the vector file....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...select the components they want for each module, generate the vector file, and quickly clone their vector...

  16. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the...means researchers who use VSV-G can deliver their genes of interest to a variety of cells and study their...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

  17. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...sequences. Cas9 is a very commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce...’t cut DNA (dCas9, for example) that are used to increase or decrease expression of a gene. Screen: A ...of gRNAs with a CRISPR nuclease target multiple genes in a cell population. The resulting mutant cells...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...

  18. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor... Sign up to write for the Addgene blog! To sign up to write for the Addgene blog simply email us. We can...write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some topics you might...Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your name out there!...include on CVs and Resumes. An Expanded Network - Addgene will promote your blog on a variety of social media...

  19. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ... what part of a gene enhancers like to interact with, and how many promoters or genes each enhancer is...plot line in gene regulation. When chromatin conformation data is compared with changes in gene expression...dynamic role in gene regulation The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE...changes in gene expression and determine who’s a key driver of these changes. By generating a more vivid...Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions...updated Jul 2, 2020. Promoters may be the star of gene regulation, but enhancers and chromatin looping ... bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers are located ...

  20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added ...-based genome engineering has revolutionized the gene editing field by making experimental workflows considerably... tools. As a consequence, a critical step in the gene editing workflow - analyzing the data - is often...likely to result in a functional KO of the targeted gene.   Analyze Complex Edits   Traditional Sanger sequencing-based...result in a functional Knockout (KO) of the targeted gene. The analysis can be sorted by any of the parameters...validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please contact us at [email protected]...
Showing: 161 - 180 of 250 results