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...researchers around the world. We take this responsibility seriously. Every decision we make, every project... in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there ...

...protein that undergoes a conformational change in response to the molecule it detects. This change then generates...with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent...

...often driven by the AAV p5 or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small...gene which differ between the AAV serotypes are responsible for serotype-specific differences in tissue and...

...absence of the correct wavelength of light. This response is immediate, on the scale of milliseconds, and...result, it is not possible to elicit an immediate response or track the exact time of activation. Receptor...

...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing DNA: temperature...

... Clandinin lab used SPARC to image the calcium response in the dendrites of T5 neurons by placing the ...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...

...efficiency is consistently higher than that of H840A, especially with smaller nick distances. Using D10A, they...strategy can expand the targetable region, which is especially useful if there are no available guides close...

...plastic is a substantial portion of lab waste. Despite the fact that researchers represent a minority ... already leading the cultural change toward responsibility in our lab’s environmental impact, but we can...

...associated data to be able to use them effectively. In response to the constant evolution of scientific tools ...and unlock new tools and discoveries. We also responded to your feedback. Many of you asked for a smaller...

...Cas protein specifically recognizes its folds, especially the repeat:antirepeat duplex. Cas12 recognizes...potentially halves the cost of a gRNA. This is especially relevant for high-throughput applications requiring...

...epigenetic effectors. The diversity of tools is especially exciting, including histone acetylation by p300...and Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional...

...This terrible disease causes the patient to, in response to tissue damage, have excessive bone growth until...patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family...

...corrective feedback. A manager must be able to respond to the employee’s level of discomfort appropriately...with the manager as with the employee. This is especially true if the problems are large enough that the...

...have demonstrated skill at managing and this is especially true in science. It is assumed that if you are...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...

...want to choose a research question that people (especially you!) care about. Have you critically evaluated...have done recently that has been a big hit, and especially look at the new tools and methods that have been...

...outcomes of interest, and stimulate or manipulate responses through neurological means like electric stimulation... They observed increased vagus nerve firing in response to both of these stimulations. The best part? ...

...more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial cultures....org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito D (2007) Improved recombinational stability of...

...replacing its dimerization domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light... Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat Chem Biol....

... is positively regulated by RNA or proteins, respectively. A plasmid's copy number has to do with the ... contribute as well. These considerations are especially useful to keep in mind if you are planning to...

...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...said CZI Head of Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution...