Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 294 results for: expression vector

Showing: 161 - 180 of 294 results

  1. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient knockout...model in vivo. In this screen, we used the pRDA_526 vector to knock out 30 genes putatively involved in CD8...robustness of the system and utility of the pRDA_526 vector. Moreover, the screen identified putative novel...

  2. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode to each to make...cDNAs) representing core nodes that, when over-expressed, constitutively activated or inactivated the pathway...other researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene...

  3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...bacteria that have taken up this vector, but it won’t tell you whether the vector contains the gRNA insert or...X-gal allows you to identify cells that took up the vector and distinguish between plasmids that contain the...𝛽-galactosidase. Complementing this mutation by expressing this deleted portion, for example on a plasmid...functional 𝛽-galactosidase. Blue-white screens rely on vectors where the gene of interest is inserted into the..., you will see two bands: one representing your vector backbone and the other representing the insert....doesn’t contain the insert, you will just see the vector backbone band. Or, you can choose an enzyme that...specific to your insert or by using primers that are vector specific but amplify a potential insert. If you...

  4. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide  ...producing infectious virus (similar to lentiviral vectors) because they do not encode all of the necessary...plasmids allows researchers to partially control expression levels of each gene by altering the amount transfected...RNA polymerase gene or using cells that stably express T7 (e.g. BSR T7/5 cells) (1). We hope that minigenomes...on the Addgene Blog Read Other Posts on Viral Vectors Check out Our Scientist's Guide to Ebola Contribute... viral vector...

  5. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression cannot be identified...upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin with the...origin with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by...resistance markers, promoters, and inducible gene expression systems in bee gut microbiome species such as... or a bacterial microcompartment protein. The expression of this fusion is under control of the arabinose-inducible...Cells are first induced with arabinose to begin expressing the SAP-RFP fusion. Then, the cells are washed...

  6. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...one are in a pLVX vector background, so you can package them into lentiviral vectors if you need to. Additionally...selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...2 virus-human interaction network in which we expressed 26 of the 29 SARS-CoV-2 viral proteins in human...Western Blot for 25 constructs, and confirmed expression via mass spec for two of the remaining viral ...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges... a usable titer, or fine-tuning selection and expression in your target cell line, researchers often find...transcriptional activation system to drive high levels of expression of certain viral components; according the manufacturer...determination of the packaging limit of lentiviral vectors. Hum Gene Ther. 2001 Oct 10;12(15):1893-905. PubMed...Tips for titering your lentivirus Visit the viral vector featured topic page Resources on Addgene.org ...Guide Find lentivirus plasmids Browse all viral vectors ...

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... viral vector preparations. Addgene has used this method to generally validate our viral vector preparation...of an Addgene AAV viral vector preparation after negative staining. Empty vector particles can be identified...darker than full vector particles. This image shows that the vast majority of the vectors consist of full...many ways to quantify the composition of a viral vector solution, and generally these methods measure different...infectious titer. The infectious titer of a viral vector stock can vary based on transduction target and...typically quantified by electron microscopy of a viral vector solution. Since this technique is intensive and...over 95% of viral capsids in representative viral vector preparations are genome-containing (Figure 1).  ...

  9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...the Krogan lab at UCSF generated lentiviral vectors expressing SARS-CoV-2 open reading frames and identified...plasmids expressed 26 of the 29 SARS-CoV-2 proteins which all but one are cloned into pLVX vectors (for lentiviral...provide synthesized SARS-CoV-2 expression plasmids available for many expression systems: bacterial, yeast,...C-terminal deletion in the spike protein to improve expression and pseudotyping efficiency. This plasmid was...enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase...-CoV infection and deposited plasmids for the expression of cathepsin S, L, and B. This became a great...human cells. Addgene’s mammalian target plasmids express these and other targets such as TMPRSS2, FURIN,...

  10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our Molecular Biology Reference...yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains origins of replication... or S. cerevisiae. Students then determine if expression of the plasmid-encoded MET gene allows the transformed...cannot grow without uracil supplementation (6). Expression of MET/Met genes from the pYES2.1 plasmid is ...proteins, which can be used to confirm protein expression by western blotting in other experiments (Figure...Students then use replica plating to determine if expression of MET/Met genes from the plasmids allows the...Since the GAL1 promoter controls MET/Met gene expression, complementation should only occur when galactose...

  11. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...from other regions of the cell. By combining vectors expressing different fluorescent proteins targeting ...display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...excitatory and inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous.... The team engineered C. elegans cell lines to express wrmScarlet1-10 unattached to any other cellular...proliferation in acute myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle...different cell types. In one example, H2B-FT was expressed in murine B cells (BaF3), which depend on IL-3...screening Applications (CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter...

  12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation sequencing...the stop cassette is removed, the effector is expressed. By truncating one of the recombination sites ...calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin is required for virulence...4X CDRE repeat. In addition to showing that this vector is suitable for flow cytometry and fluorescence... single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019. https:/...

  13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...categories Expression: Select the model system for your research (e.g. Mammalian) Vector Type: What type...Addgene is currently distributing 94 plasmids expressing validated antibodies for neuroscience research...example I wanted to find a lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

  14. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing a ...utilizes custom mouse strains and lentiviral expression vectors (Table 1). The four systems Name Function...

  15. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...’ the homepage is still important. It needs to express value, clearly communicate exactly what a site ...wanted users to be able to find plasmids and viral vector tools more easily. In the redesign, the new search...One big feature upgrade? Blugene now has a full expression. You’ll mostly see a friendly smile, but occasionally...you come to Addgene to find the plasmid or viral vector for your next experiment, that you can find what...

  16. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...problems with Cas9 delivery. Adeno-associated viral vectors (AAV) are commonly used for in vivo gene delivery...SpCas9) and a gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of...packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall packaging capacity but necessitating...activation. Split inteins have also been used to express base editors. References Chew WL, Tabebordbar...HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038...genome engineering: An adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https...Editing: Strategies and In Vivo Delivery by AAV Vectors. Cell 181:136–150 . https://doi.org/10.1016/j.cell...

  17. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ...Practical Guide: Using pK-TnpB1 and pK-TnpB2 Vectors The vectors pK-TnpB1 and pK-TnpB2 are designed for plant...the digested vector. Step 4: Ligation Ligate the annealed oligos into the digested vector using your ...efficient delivery into cells, particularly via viral vectors. While viral delivery is a highly effective method...genome editors to date. We have developed multiple vector versions for TnpB-mediated genome editing, and ...delivery of editing reagents into cells. Viral vectors, because of their natural ability to infect cells...approval and public acceptance. Recently, viral vectors have been successfully used to generate gene-edited...al., 2025). However, a key limitation of viral vectors is their restricted cargo capacity, which makes...

  18. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...same strain. MazF expression is under control of the AOX1 promoter whose expression is induced by methanol...that are used to drive high protein expression in vivo. Expression plasmids are often lost without plasmid...as a counterselectable marker in the destination vector. Successful cloning occurs when the ccdB gene is...methanol. Once linearized, the delivery vector can be integrated into the genome to introduce the genetic...hok/sok system to create strains that can stably express antigens in sufficient quantities to generate an...plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene."...James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters...

  19. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...homology. Producing yeast expression cassettes Traditionally, generating expression cassettes for yeast transformation... mutations Cloning sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple...PCR to generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, ...simple as mixing fragments together with linearized vector for seamless joining of multiple mutations spread...

  20. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics Fluorescent...Sensors This system consists of a genetically expressed enzyme, typically on the cell surface, which can...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned,...
Showing: 161 - 180 of 294 results