We narrowed to 872 results for: fer

...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require...bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these...together genes from gas vesicle operons from two different bacteria, Bourdeau et al created two constructs... 1 (arg1) and acoustic gene 2 (arg2). The key difference between arg1 and arg2 vesicles is the amount ... invisible to ultrasound, which allows for differentiation of arg1 and arg2 ultrasound signals. This also...and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A., Lakshmanan...Blog Learn more about in vivo imaging tools The luciferase reporter gene The green fluorescent protein reporter...

...Direct reprogramming describes the process where differentiated cells are turned into a cell type of choice...identifier and allows cells to be tagged with different CellTag libraries at multiple timepoints during... (iEPs), a self-renewing cell type that can differentiate into hepatic or intestinal cell types. MEFs ...inefficient. Cells were sequentially tagged with different CellTag libraries at three time points during ...MettI7a1 encodes an uncharacterized putative methyltransferase. Adding Mettl7a1 to the standard Foxa1-Hnf4α...are undergoing identity changes, such as the differentiation of induced pluripotent stem cells or the clonal...visualization tool. Check out the CellTag webpage! References Biddy, Brent A., et al. "Single-cell mapping ...

...19 pandemic,” says Balazs.     References and Resources References Cele S, Gazy I, Jackson L, Hwa S-...neutralization effectiveness of current vaccines differed depending on the variants (Garcia-Beltran et al...plasmids which encode spikes found on all of the different circulating variants. This allowed us to create.... In their assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection...variants. Yet, the B.1.351 variant, which has only 9 different mutations compared to the wild-type, escaped neutralization...Garcia-Beltran et al., 2021 with permission.   Difference in neutralization after one dose and two doses..., this study was the first to compare so many different strains. It was also the first to perform neutralization...

...have risen into yeast expertise! References and Resources References Liu, Wei, et al., From Saccharomyces...applications in food science and biotech due to its fermentation abilities and other useful traits. The “biotech...lists some of the biological commonalities and differences between K. phaffii and other yeast strains.   ...aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol...Applications for K. phaffii There’s a number of different uses for the phaffii-lous strain. Protein expression...peroxisome studies since their peroxisomes will proliferate upon a media change into methanol and peroxisomal...peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation K. phaffii’s genome has...

...add loading buffer to your digest reactions before loading them. The glycerol in the buffer will make sure...based on the predicted sizes and organization of different features within the plasmid. Restriction analysis...can search NCBI for YGOI to find the particular reference sequence if necessary. The example plasmid on ...fragment having increased intensity to serve as a reference band. The uncut DNA (lane 2) shows 3 possible ...visualize the full size of your construct. Consider buffer and temperature compatibility when digesting with...versions of the endonucleases and/or supply custom buffers as means to avoid this issue. For your gel: ...in the gel well and the dyes provide a visual reference point so you can easily assess how far the gel...

...in. The differences They say the devil is in the details - and that’s where the differences in these... RNAi resources and go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D....? If you’re thinking, ‘wait, those terms mean different things?!’, you aren’t alone! We’ve noticed a lot...selections The common goals The confusion in differentiating between a screen and a selection isn’t surprising... you would individually treat cells with many different drugs and then perform an assay to look at an ...enhancer/suppressor screens. Selections Selections differ from screens in that only survivable manipulations...experimental question. Now that you understand the difference between the two, it’s time to check out our CRISPR...

...skills that were quite different from mine. Learning to bridge those differences was a pivotal moment in...technical problems in the lab, management involves a different skill set: mentoring, setting strategic goals,... day-to-day details. Ignoring individual differencesManaging a team isn’t “one size fits all”. Each team... needs. For instance, I had one colleague who preferred detailed instructions and regular check-ins, which...autonomously. Failing to recognize and adapt to these differences could have led to frustration, micromanagement... particular, served as a great "baby project," offering a supportive environment to build my management...noticed tension between two team members with differing approaches—one detail-focused and the other big-picture...

...imaging for advanced monitoring of different factors, in different or the same cellular location, all ...covered with packaging plasmids! References and Resources References  Bhalla N., Jolly P., et al. Introduction...biosensors provide quantifiable readouts for levels of different chemicals, hormones, proteins, etc., that you ...as calcium. This varied component, which we will refer to as the analyte, needs to be specifically recognized...and zinc. These ion sensors can be targeted to different cellular locations or tissues depending on the...acceptor of a FRET pair on actinin will produce different signals based on changes to the structure of actinin...organisms, but AAVs make it possible. In AAV systems, different serotypes can be used to affect or determine viral...

...antibodies differ from other types of antibodies? How monoclonal antibodies are made “Monoclonal” refers to ...for your experimental needs.     References and resources References Bradbury ARM, Trinklein ND, Thie ...experiments, you may be curious about all of the different kinds of antibodies that are available. One common...heterogenous mixture of antibodies that recognize many different epitopes on the same protein - are more likely...to produce monoclonal antibodies. Genetic drift refers to changes in the nucleic acid sequence of the ...impacts the specificity, avidity, and number of different antibodies produced by the hybridoma. Image from..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...

...excitatory and inhibitory channels activated at different wavelengths of light (Vierock et al., 2021). Step-function...stay on until a second pulse of light, with a different wavelength, turns it off, allowing the cell to... secondary signaling pathways. There are many different opsin (natural and engineered proteins responsive...pathway to activate various protein regulators at different points in the pathway. Photoswitches are great...successive steps along a pathway, there can be very different responses to long vs. short stimulation times ... & Cui, 2015). You’ll need to test for these differences when designing your experiment.  Finding the ...available to help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (...

...’s of working in different sectors. Luckily, I have worked for a variety of different organizations, so...overview of my experience and how I felt about the different positions to enable them to decide for themselves...effectively. I had to learn how to cope with a different climate and culture, and also needed to be resourceful...very quickly; as soon as I learned how to do the different tests and became efficient, I then started to ...diagnostic tool for the detection and diagnosis of different types of pancreatic cancer and highlighting which...were keen for me to become involved with many different aspects of the company and gave me the freedom... and travel to meet scientists, making my job different every day. I love the freedom to design and develop...

...international mentoring conference held at the University of Valencia (Spain). At this conference, mentors and mentees...professional development and networking resources offered by programs like IMP USA-Europe not only provide...international mentors. Universities from five different geographical locations in Spain were enrolled:...mentees held scheduled monthly progress video conferences and had real-time correspondence via e-mail throughout...Mentorship Program and the International Mentorship Conference In addition to a pre-program self-assessment ... exchanged impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary...community overseas. Official representatives from different Spanish universities and high level public and...

... interest is then eluted using a high imidazole buffer that outcompetes the interaction between the protein...contaminating protein (orange). A low imidazole wash buffer disrupts the weak interaction of the contaminating...the protein of interest. A high imidazole elution buffer disrupts the interaction of the protein of interest...step to remove the tag in order to prevent it interfering with protein function. In contrast, the polyhistidine...The concentration of imidazole used in the wash buffer should be high enough to disrupt contaminant-metal... of a protein. Neither the N or C terminus is preferable over the other however, tag placement can affect... it is, and the greater the risk that it will interfere with protein function.  Additional Benefits of...

...ganglia in movement disorders.   References and Resources References Armbruster BN, Li X, Pausch MH, Herlitze... be the best option. Fluorescent proteins can interfere with fused protein function Under some circumstances...found that the fluorescent proteins can, indeed, interfere with the correct function of the protein of interest...in monkey neurons, we next hypothesized that a different tag fused to the hM4Di might improve the transport...Surprisingly, we found that this small change made a big difference. Replacing the mCherry with the HA tag resulted...transgene of interest. In our studies, mCherry interfered with the transport to the membrane of the DREADDs...doi.org/10.1002/0471140864.ps0909s73 Additional references on the Addgene blog Browse Fluorescent 101 blog...

...there’s less malignancy because there’s less proliferation. However, the lack of caveolin 1 in breast cancer...growth mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread... integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain... (micro RNA) and other molecules that can be transferred to other cells, inducing epigenetic changes in...regulates transcription. Without TKA1, cells proliferate and cannot undergo apoptosis, thus multiplying...issues. Follow her on Twitter @BrbaraPinho6.      References Allinen, Minna, et al. "Molecular characterization...

...were also tested in different plasmid backbones that varied in size or carried different genes. In these ...expression can make data hard to interpret or can interfere with the precision needed in metabolic engineering...ranging from 3-100 per cell, a stabilized promoter buffered against the effects of copy number on reporter...number. Moreover, gene copy number is affected differently by growth rates when a gene is expressed from...Segall-Shapiro et al. showed nearly no position-dependent differences in gene expression. On the other hand, slow growth...promoters can eliminate variability between the different components. When your pathway needs precise fine-tuning...levels, give these stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag...

...situ hybridization (FISH) and chromosome capture suffer from low resolution and can’t be used in vivo. ...NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three distinct colors...orthologs is key: since each ortholog requires a different PAM sequence, a gRNA designed for one dCas9 should...specific for telomeric sequences and showed that different fluorescently labeled dCas9s are efficiently directed...target sequence. They succeeded in labeling two different pairs of chromosomes using gRNAs specific to sequences...specified by PAM sequence, each color requires a different Cas9 ortholog, and the target sequence must be... green, and red. In contrast, a gRNA with two different hairpins will produce a secondary color cyan, ...

...Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows researchers...whenever you hear it (whether at a scientific conference or a karaoke bar), you probably want to sigh....demethylation?  Dividing cells depend on DNA methyltransferase 1 (DNMT1) to copy methylation marks from parental...demethylation – tight binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9... CRISPR-based tools for epigenetic editing.   References and resources:  Additional Resources on the Addgene...Addgene.org Read our CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) ... al (2018). Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun 9, 597. https://doi.org...

...as they are often quite different from one library to another. They also differ from the CPS process in...examine three things: Aligning the NGS result to a reference sequence to confirm backbone elements.   Confirming...deposited plasmids is to align the NGS result to a reference sequence like a known backbone sequence. We’re...through BLAST or by direct alignment to an NCBI reference sequence or a sequence provided by the depositor...synthesized regions that have no publicly available reference sequence or plasmids containing genes with many...features using Snapgene. If we find information that differs from what we would expect given the data provided...the depositing laboratory confirms that these differences are expected and do not affect plasmid function...

...of the Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’...Thoughts on Guangzhou and the Science City The conference was located in the modern, relatively new “Science...national science and technology park, it enjoys preferential governmental policies for a high-tech development... which I could identify. There were dozens of different mushroom varieties alone. At least I could tell...requires an interdisciplinary team.  Day two of the conference was kicked off by Nobel Laureate (and Addgene...circRNAs do yet or how they do it. Of course, No conference would be complete without CRISPR Michael Terns... also exploring the use of cas9 proteins from different species for genome engineering. The final keynote...