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We narrowed to 256 results for: human dat

Showing: 161 - 180 of 256 results

  1. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...to Improve HDR Efficiency for CRISPR Editing in Human Cells Hands-on tips for repair template design from...out #KSgenome on Twitter if you missed our live updates! My most popular tweet from the conference is...bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current with the latest and...

  2. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org/10.1089... and Peripheral Nervous System and Relevance to Human Gene Therapy. Front Mol Neurosci 13. https://doi.org...to ensure the accuracy and reproducibility of the data and can instill greater confidence when interpreting...

  3. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...peer-reviewer, you must never forget that there is another human who is reading your comments. Receiving a peer-review...share a wealth of data on ... The language is ... The general representation of data is... The figures...tools for data analysis/visualization using R/Shiny. She is passionate about open science, BIG data analysis... your peer-review Additionally, you can ask that data and resources described in the manuscript are accessible... journal you are reviewing for. The code and the data can be deposited in the public on-line repository...

  4. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...adapt and embrace new ways of working, reconnect as human beings and colleagues, eventually encouraging purposeful...scientist at Weill Cornell Medicine. Discussing data with colleagues can be more difficult during the...as a break, but can also be used to brainstorm on data in smaller groups, where for instance the presence...meeting where all lab members talk no science. No data presentation will appear here, and only general ...upstream a career from academia to industry”. To date, aside from doing science, he has assisted many ...

  5. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Around 20% of human genes have no known function or a poorly defined function (Wood, et al). In the microbial...  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids 101 e-book ...

  6. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...Louten, J. (2016). Virus Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-...Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral Protocols...

  7. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...medium during adeno-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475...production strategies or developing novel methods for data analysis, our team’s goal is to provide scientists...

  8. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Oliver Sacks examines the relationship between the human brain and music: how we perceive it, why it affects...of southern England. While the length may be intimidating, Ruthefurd’s writing will capture the attention...favorite books” to “I barely got through it.”  To date, we’ve read 76 books for ABC. Some are easy reads...

  9. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...match variations present in the data such as synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching ...processes to curate the data our depositors share and enrich it with sequencing data; as a result, the materials... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist, and Software... menu. For users who prefer to use search, our updated search engine is now more powerful, intuitive, ...knowledge of the scientific domain in general and of our data in particular and our deep understanding of Addgene...and synonyms of the same term that appear in our data into a single filter value. For example, there is...

  10. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...a 9 amino acid peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively... brought about by the tag so it’s important to validate tagged protein functionality (Arribere, et al)...

  11. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...Richie lab are especially useful for studies in non-human primates, where fusion fluorescent proteins can ...quality control. For an estimate on availability date or to be notified when a particular catalog item...

  12. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...re-amplified our distribution stock of the Brunello Human gRNA library, and we thought it would be a good ... Topic Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries...

  13. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...0.66 million targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely.... Ghorbal, Mehdi, et al. "Genome editing in the human malaria parasite Plasmodium falciparum using the...separate sgRNA design tools into database and de novo solutions. Database tools allow us to view and get...a good example of how database tools can be used to aid design. These databases are still young and will...usually the target site. Multi-locus asseEENdb database ssment would be required to fully evaluate off-targeting...rational design. The first of only three such databases, EENdb (published 1 Jan 2013, no longer available...catalogue of reported sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy...

  14. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation ...complications of diabetes Gloria Otito Izu, PhD Candidate, University of Ibadan, Nigeriaunder supervision... of Ibadan, Nigeria Gloria Otito Izu is a PhD Candidate working under the supervision of Akindele Oluwatosin...projects progress. Subscribe to the Addgene blog for updates and future collaborations. Many thanks to all of...

  15. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ... for expression in Cre-expressing cells hSynap: Human Synapsin-1 promoter. Good for neuronal expression...Ford in 2018. It was updated by guest blogger Abhi Aggarwal in 2022.  Recent updates to iGluSnFR and SF-iGluSnFR...SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here, we look at the origins...

  16. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...choose a lab that works on problems pertinent to human disease. For more details and many more suggestions...needs. These two factors will provide a solid foundation on which to thrive during time spent as a graduate...also includes a useful list of questions for a candidate to ask lab heads, lab members and lab neighbors...watch the webinar "How to Choose Your Next Lab" (updated 2020 version) by Addgene Executive Director, Joanne...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...our users, we now provide the Brunello (targeting human genes) and Brie (targeting mouse genes) pooled libraries...with all of our viral preps and host our expression data (western blots or fluorescent microscopy) on the...these widely studied signalling pathways. Find Validated gRNAs to use with your Cas9 Virus Pooled libraries...

  18. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...of technologies to functionally investigate the human genome. Doench says he enjoys working on technology...still gets to see data as it’s coming out and says “That first glimpse of what the data look like... when...

  19. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...in metazoans. The superfamily is comprised of 48 human and 49 murine members. NRs permit the integration...coregulator-related global gene profiling datasets in to our data mining tool, Transcriptomine. We are currently...links to datasets and reagents in the field. Additional information can be found in the Datasets and Reagents...research, and has generated a set of comprehensive datasets that collectively address many important and fundamental...nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the expression patterns...seeking to compliment these consortium-generated datasets with a systematic effort to compile NR- and coregulator-related...

  20. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...opportunities. In an age of scarcity, the rational human being will tend to clinch every opportunity for ...resources, we created STEAMid, for a nationwide database of  STEAM internships for high school, college... a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-3 individuals in your...applications. Thus these noble individuals are inundated with requests. The easier you make it for them...Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo...
Showing: 161 - 180 of 256 results