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...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...

... Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on Addgene.org... Hot Plasmid Pages to See the Latest Plasmid Technologies ...

...knockout of genes in the immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590...subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590...

... curator of the thousands of amazing plasmid technologies contributed by our depositors, Addgene strives...certainly not least, we often feature new plasmid technologies on our blog and encourage you to fill out this...

...reported doing in a paper that appeared in Nature Biotechnology back in 2011 and then in another Nature Methods...protein for in vivo imaging. Filonov et al (Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.) Near-infrared...

...intertwined. In this blog we will review how CRISPR technology has brought drug and genetic screens together...of control have also been added to the CRISPR technology to allow time-controlled edits to occur, such...

...rapidly generate stable transgenic cell lines. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. PubMed... modify clinical-grade T cells to target CD19. Immunol Rev. 2014 Jan;257(1):181-90. doi: 10.1111/imr.12137...

...field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled "Advances...amazement at how much has been done with CRISPR technology in just 2 years and excitement about how much...

...Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid...

...more standardized methods for evaluating CRISPR technologies, including the ever important on/off-target ... are aimed at improving translation of CRISPR technology, but they would also bring benefits to researchers...

... Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...

...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833...

...female. HEKs are generally cultured as an adherent monolayer, though derivatives have been adapted for growth...Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389/fbioe.2021.796991...

... development, vaccine development, and assay technologies makes her a perfect fit for Addgene. She was...experience, soon directed himself to finding “immunological and virological solutions to crises like those...

...at all levels.      Jargon is field-specific terminology or language. This can refer both to terms that...that are only used in the field and terminology that is widely used but has a very specific use-case (definition...

...Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative. Our first collection ...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554...

...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating...

...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection...

...our network.  We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology...

... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen biology to treat...