We narrowed to 440 results for: pes;
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TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub reports detailing antibody...can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility. But this...
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A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....perturbations in several genes spanning 33 cancer types. The types of mutations they identified included gene... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....genes have different functions in different cell types, at different times, and in combination with different...L-CHIME Knocks out a single gene in defined cell types (lineage-based) pXPR_070 Violet-excited GFP ... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog PostPublishedOct. 15, 2024, 1:15 p.m....repository and their choice of five different serotypes. Figure 1: The process of requesting...service. At launch, we will offer five different serotypes for packaging: AAV1, AAV2, AAV5, AAV8, or AAV ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m.... continue to improve plant nutrition, yield, and pest resistance. Much of plant genetic engineering revolves...DNA yield and transformation efficiency in both types of bacteria. They also replaced the promoter regulating... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....and freely behaving mice to gather the following types of data: Report time-course and concentration of... and freely moving mice to gather the following types of data: Report endogenous DA release from a single... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified: AAV1-11. Nine... in the cap gene which differ between the AAV serotypes are responsible for serotype-specific differences... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....technology is versatile and can be applied across cell types and organisms by replacing the transcription factor... allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase can work in other... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....access unique epitopes with high affinity, due to a greater ability to target concave epitopes Suitability... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....a marker for the mutation. When designing these types of experiments it is best practice not to use the...compatible! Check out this list of common E. coli genotypes and their natural resistances. Additional Resources... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....drum. They have a wide variety of possible genome types that can be either double stranded (ds) or single...them far safer to work with than virions. The four types of viral vectors (which many researchers will informally... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....antibodies within certain classification groups called isotypes. Why does isotype matter? Isotype matters when...secondary. If you were to change one of the antibody’s isotypes, however, you could then use 2 different secondaries... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....deliver a genes of interest to a variety of cell types in-vivo. These are widely used by the optogenetics...revolutionized how researchers make knockouts and other types of genome edits, but, even with CRISPR, it isn’t... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....re also distributing AAV preps with particular serotypes that can be used to deliver fluorescent proteins...future users. We’re excited to be distributing new types of materials. At the time of publication we’ve already... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....there is a very real risk of mixing up samples, serotypes, or both. Unfortunately, the standard AAV QC regimen...References Berns KI, Adler S (1972) Separation of two types of adeno-associated virus particles containing complementary... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression... to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV inventory for the... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m.... et al. tested iTOP in a series of primary cell types using Cre and various loxP reporters. In both mouse...primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal and gut... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....comparing the transduction patterns of different AAV serotypes. To help make this easier for scientists, we now...testing. These samples are available in various serotypes of two popular control AAV encoding GFP. Check... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the...blood-brain-barrier. In summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....Postdoctoral Research Fellow with Pamela Silver. Most types of biological motion (whether endocytosis, vesicle...explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis, vesicle...