We narrowed to 701 results for: plasmid

The Addgene Blue Flame Awards celebrate depositors with highly requested plasmids. Learn more about the awards here.

Nov. 14, 2016, 3:30 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Feb. 7, 2017, 3:30 p.m.

FastCloning is a fast and easy technique that can replace restriction cloning. Learn about this great plasmid cloning technique here.

Nov. 1, 2016, 2:30 p.m.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

May 15, 2014, 3:33 p.m.

Learn more about luciferases, a class of enzymes used in bioluminescence, and how they are commonly used in reporter plasmids.

July 22, 2025, 1:15 p.m.

Check out some great blog posts from Addgene Depositors.

Nov. 18, 2016, 3 p.m.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

May 6, 2025, 1:15 p.m.

Learn how Addgene works with you to increase your work's visibility and citations through efforts to organize and curate the repository.

March 10, 2016, 3:30 p.m.

Learn about the latest statistics on CRISPR plasmid deposits and distributions and how this reflects the broader CRISPR community.

Aug. 6, 2026, 7 p.m.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

Aug. 2, 2016, 2:30 p.m.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.

CRISPR is a story of revolutionary science - but it's also a story of community and sharing. Addgene shares our part in this story.

April 18, 2024, 1:15 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Synthetic origins of replication, or ORIs, from the Chappell Lab have improved plasmid compatibility and copy number control compared to natural ORIs.

April 14, 2026, 1:14 p.m.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

We're updating our Hold for Publication policy. Read about how these changes will help streamline our workflows and get your plasmids online faster!

June 24, 2025, 1:15 p.m.

Use this transfection calculator when packaging your AAV vectors.

Jan. 23, 2018, 2:04 p.m.

This post highlights some of the tools Addgene makes available to industry scientists.

Jan. 13, 2026, 2:15 p.m.