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We narrowed to 388 results for: plasmid dna

Showing: 161 - 180 of 388 results

  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...editing of a target base in genomic DNA without double-stranded DNA cleavage." Nature 533.7603 (2016): ...Programmable base editing of A• T to G• C in genomic DNA without DNA cleavage." Nature 551.7681 (2017): 464. PubMed...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...frequencies and relies on the efficient delivery of donor DNA to facilitate repair. Since the development of CRISPR-mediated...Cas9 nickase (nCas9). nCas9 creates a nick in the DNA by cutting only a single strand, dramatically reducing... detrimental indel formation. After binding with DNA, the CBE deaminates a target cytosine (C) into a ...this change. However, there are no naturally known 'DNA adenosine deaminases' which would theoretically give...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything needed...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ...backbones New recombinant antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer... swap sequences effortlessly.  We built these plasmids for flexibility and speed, so you can design, ...

  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...other can the DNA strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...Tagged protein can easily be overexpressed from a plasmid but overexpression may interfere with the system.... Both antibodies have a short antibody specific DNA strand added to them. Only if the proteins of interest...

  4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...chromatin, the complex of DNA and proteins that forms chromosomes. These long range DNA interactions are often...digest cuts DNA into smaller pieces, and proximity ligation creates chimeric circles of DNA that are distant...CAPTURE method. After protein and DNA complexes are cross-linked, DNA is digested into smaller pieces. ...ligated together to create loops of DNA. This is key to linking pieces of DNA that are far away from each other...important supporting roles. Enhancers are cis regulatory DNA sequences that, when bound by transcription factors... looping with the photo “developed” by analyzing DNA sequencing data. While “pictures” generated with ...picture” is then developed by detecting these chimeric DNA fragments by PCR or next-generation sequencing. ...

  5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...to defend against both DNA and RNA viruses - 2 cas systems regulate at the DNA level and 1 at the RNA ... Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety (see slides...is in one of the top 10 countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic...Opening talks – Genomics discoveries and moving beyond DNA Eric Green, Director of the National Human Genome... inheritance that might be less error prone than DNA replication. He too mentioned how “surprising” small...ribosome is in a complicated conformation “not like DNA which has a boring structure.” That certainly summed... dna...

  6. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...fluorescent protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...classrooms, like gel electrophoresis to separate DNA or transforming cells with green fluorescent protein...ll work with you to get the word out about your plasmids and their potential educational uses. We believe...30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional ...developmental biology Use the pathways over time plasmids to engage students in functional genomics research...out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here ...

  7. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...thermocycling program. One key difference is the plasmid DNA must be released from the bacteria in order to...just need to lyse the bacteria to release the plasmid DNA by either briefly boiling the sample before use... sequences of the junctions between the plasmid and insert DNA. Colony PCR will greatly reduce the number...the correct orientation or even if it’s in your plasmid backbone. Backbone-specific primers: A second...orientation of your insert or if the insert is in your plasmid Have to make a new primer pair for each insert ...about the size of the insert and if it's in the plasmid Can be used to screen clones that were created ...created with the same backbone because primers are plasmid dependent Doesn't provide information about the...

  8. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...transfer plasmid, which contain regions of dsDNA. To generate a 3’ hydroxyl group needed for DNA replication... AAV.  Where do you start? Plasmids of course! You just need three plasmids to start making the AAVs you...packaging plasmid which contains the AAV structural and packaging genes, the adenoviral helper plasmid which...packaging plasmid and take a look at each of its parts. Key elements of the AAV packaging plasmid The packaging...packaging plasmid aka the RepCap plasmid encodes two genes: the replication (rep) gene and the capsid (... AAV RepCap plasmid! Check out this blog post to learn more about the AAV transfer plasmid! References... for the virus to replicate,  and the transfer plasmid which contains the viral genome. In today’s blog...

  9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...) ChiRP-seq RNA Hi-C DNA (genome-to-genome interactions) DNase-seq DNA (regions that are accessible... regions of DNA that demonstrate “spooky action at a distance.” Through the marvel of DNA compaction, ...details on DNA compaction and the structural proteins that make it possible, see the appendix DNA is a 3D...See below for further details. DNA is a 3D Fractal Figure 2: DNA compaction. Graphics courtesy ...For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome, ten...coordinates for genetic elements like protein, RNA, and DNA (Table 1). As with GPS in the real world, coordinates... examine how we can locate powerful stretches of DNA known as enhancers with the help of genome math. ...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...find all of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter...Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources at ...

  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...based on the functional elements they contain. Plasmid DNA in both prokaryotic and eukaryotic systems must...Download our Plasmids 101 eBook to get all the background you need to start working with plasmids! What are...Techniques Read all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...Rho-dependent terminators are not usually employed in plasmid-based expression systems, so these will not be ...the end.  Nearly all common bacterial expression plasmids use Rho-independent terminators, which include...the right. The tertiary structure of the hairpin-DNA complex is thought to destabilize the transcription...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging...the ability of the endonuclease Cas9 to create a DNA double stranded break (DSB) at a genomic site specified...homology directed repair pathway, a new user-defined DNA sequence (like GFP) can be inserted at the DSB. CRISPR...The Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR...the user first designs either oligos or synthetic DNA for the desired gRNA target sequence, as well as ...repair template. SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent...tag and homology arms). Digestion of the donor plasmids with SapI allows the tag, selectable marker, and...

  13. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ...identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when over-expressed...cloned all the cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode to each... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer...Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on...curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them into lentiviral...

  14. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...designed so that the single stranded DNA folds back on itself to become dsDNA in the nucleus and is a good option...Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid...repeats – the sequences that flank the deliverable DNA) are removed, resulting in recombinant AAVs (rAAVs...cell machinery, and synthesize a second strand of DNA before expression of their cargo can begin, which...not divide and replicate in the same way the host DNA does. Because of this, fast dividing tissues will...differentiated, non-dividing cells.   Figure 2: DNA rescue and transduction of a conventional single-...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....

  15. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...intermediary DNA replication step between DNA and mRNA. DNA genome replication can involve an RNA or dsDNA intermediary...Louten, 2016 Viruses with DNA genomes can either follow central dogma (gDNA to RNA to proteins) or require...retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before being...replicate their genomes using a variety of RNA or DNA intermediaries, depending on the class of virus. ...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors...double stranded (ds) or single stranded (ss) (ie, dsDNA, ssDNA, dsRNA, positive sense ssRNA, or negative sense...

  16. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ... presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing cell...cell must replicate the plasmid in addition to its own chromosomal DNA, costing additional resources to ...to maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once...Biology Reference Pages View our Plasmid Protocols Find Your Perfect Plasmid Backbone ...think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient... which cells successfully received the correct plasmid, scientists would spend hours to days trying find...once – it allows a scientist to easily detect plasmid-containing bacteria when the cells are grown on ...

  17. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection pages... resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model...using the fruit fly far before we even knew that DNA was genetic material (Jennings, 2011).   The fruit...processes with our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast...Scientists can simply inject one-celled embryos with DNA or RNA to edit their genomes or to create transgenic... pancreas. They first tagged these cells using plasmids. Then using HOTcre, a method that uses heat induction...Transgenic animals can be made by simply micro-injecting DNA into the worm, such as animals that express fluorescent...

  18. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... inosine from DNA: N-methylpurine DNA glycosylase (MPG), also known as alkyladenine DNA glycosylase (AAG...N-methylpurine DNA glycosylase / alkyladenine DNA glycosylase  Removes hypoxanthine from DNA, creating an...create an abasic (empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes...(AP) site. UNG / UDG Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic site. MPG...W. (2024). Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase. Nature Communications...fused to nCas9. First, MPG removes the G base from DNA to create an abasic site. Like in most other base...non-edited strand to encourage the cell to repair the DNA using the edited strand as a template — in this case...

  19. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types ...Scientists eBook Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career... two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the 3rd edition has now been released...top of the many developments in science and the plasmids, technologies, and researchers behind them. As...most popular posts continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to ... the background they need to get started using plasmids and the revolutionary CRISPR technology in their...

  20. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... to make my own glass milk for purifying cut plasmid DNA from agar slices.  Molecular biologists used ...daily before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey... I realized I was pouring large agarose gels for DNA visualization or isolation, then using only a few...bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice, I got ...
Showing: 161 - 180 of 388 results