We narrowed to 187 results for: rras

1. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ...non-sequence specific frameshift mutations through aberrant double strand break repair, it has proven more...

2. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

   Type

   Blog Post

   Published

   Sept. 1, 2020, 1:15 p.m.

   ...Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective ...

3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ... to better capture these interactions in a vast array of biological contexts.  Proximity labelling relies...

4. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...target common IgGs and then conjugate them with an array of fluorophores. It is common for labs to keep a...

5. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   Published

   June 23, 2016, 2:30 p.m.

   ...expansion of studies on protein phosphorylation in an array of model systems coupled with advances in mass spectrometry...

6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...functional Cas9 nuclease, so there’s no potential for aberrant DNA cleavage. Direct HIV-1 cleavage allows T-cells...

7. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan...

8. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   .../doi.org/10.1073/pnas.1936192100 Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated...

9. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ...Dumitras, C., Gong, B., Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019)...

10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...firmly onto each to remove cell debris. Then, UV-irradiate the membranes for 15 min to kill any remaining...

11. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering...

12. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...

13. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...project to validate some genomic changes, so this arrangement was ideal for everyone.  The cutting edge science...

14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...University Miguel de Cervantes, the University of Navarra, and the University of the Basque Country. Mentees...

15. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...therapy 8.16 (2001): 1248. PubMed PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is...

16. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...the Output_Short file directly. We have used CustomArray, Inc for this purpose.  The gRNA oligos are...

17. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...perfect combination. The EcoFlex Kit has a wide array of DNA parts that can be assembled into individual...

18. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...for error-free knock-in at both alleles may be warranted (20-30% of all tagged clones were bi-allelic in...

19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...100 Å). Researchers have developed an extensive array of chemical labeling strategies suitable for targeting...

20. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...Cas9 grips nucleotides 1-10 of the guide RNA, prearranged for base pairing with a complementary DNA strand...