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...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...library that contains up-to-date information and data-sets, as well as an interactive gRNA viewer. The Moffat...address this problem, Csaba Pál’s lab has created a set of vectors that allows one to use the MAGE method...method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase... kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository. The... unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement unused auxotrophies...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...

...machine learning approach, the lab proposed multiple sets of Cas9 mutations that conferred new PAM preferences...mutations produced efficient editors, the Kleinstiver set performed best when paired with the V106W mutation...

...extracted, sectioned, and imaged, with all image sets available at the Allen Institute’s Genetic Tools...Enhancer Collection is a quick guide to a smaller set of tried-and-true winners, containing enhancers with...; font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9...

...Goedhart and colleagues in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1...controlled phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and...

...specifically developed to thrive in laboratory settings. One of its most attractive features is its stability...easily maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms...

...in leukemia, the Lowe lab was able to identify a set of mutations that made cancer cells more resistant...systems check out Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally ...

... go over the details step-by-step. 1. Digestion Set up restriction digests for your insert (or donor ...of recipient plasmid to insert. It is critical to set up a negative control ligation reaction with no insert...

...approaches to visualize bacteria in an educational setting. The Symposium on ‘Creative Approaches to Presenting...

...For each of these 17 pathways, they identified a set of one to three mutant complementary DNAs (cDNAs)...

...that will put you in position to pursue a diverse set of career paths. Check out Joanne's Reddit AMA Here...

...Elegans Kit - Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested...

...Amy say is the quickest way to get things started? Set up your own Science by the Pint series and organize...

... part of my job, I travel around the country and set up meetings with PIs to discuss the benefits of depositing...

... questions for specific feedback in non-public settings. Ask over coffee.  Have regular 1:1 meetings with...

...think I should talk to?” That way you are always setting up your next opportunity to learn. Additional ...

...Google Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders....

...laboratories remains limited. Members of the Stanley Qi Lab set out to address this limitation by developing CRISPRkit...inexpensive system for CRISPR experiments in classroom settings without specialized equipment (Collins & Lau et... et al. (2025) under CC BY license. These datasets (in healthy mice and a Huntington's disease model...

...and Ioannidis 2005) and changing an experimental set-up partway through an experiment in an attempt to...a journal prior to performing the experiment (or set of experiments), the design would be reviewed, and...biological researchers deal with larger and larger datasets and use computer based analysis techniques, an...

... produce a BRET probe for imaging. These authors set out to design a better fluorescent protein that could...could be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...impractical or infeasible for certain experimental setups. The brightness of NLuc has caught the attention...

...fusion protein allows for LSD1 targeting to a wider set of genomic targets. These two vectors are part of...may make sense to try multiple CRISPR tools when setting up your experimental system. Let us know about ...