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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 459 results for: try

Showing: 161 - 180 of 459 results

  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...example where a selection would work is if you are trying to identify genetic conditions which convey resistance...

  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...next section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational... to other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in ...

  4. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...originally came about as Bhang and co-workers were trying to develop a way to quantify the take rate of mouse...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...

  5. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...toolbox you are putting together?  Voigt: We’ve been trying to get everything to the scale of building synthetic...is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth. Something has to be...program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the moon was built on...We did demonstrate that you can use the CRISPR chemistry for a NOR gate. But there are a couple of problems...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...could bind not only the endogenous proteins you are trying to block but your primary antibody as well.  Next...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...Other Conjugates to Know It would be overwhelming to try to cover all of the possible antibody conjugates ...like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And, you can also conjugate...purposes.  Coupling up - antibody conjugation chemistry  Whatever the conjugate, the molecules must somehow...Chattopadhyay PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...site. Or, if you are visiting a local classroom or trying to explain your science to the general public, ...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...depends on several factors: The protein you are trying to express How much protein you need   Your plans... its use as a model organism in genetics and biochemistry. When using S. cerevisiae, researchers often...

  17. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...getting sub-optimal plasmid yields from your prep, try our tips below! How to optimize your plasmid prep...

  18. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... also be determined empirically but it’s best to try those that have already been published first. Because...cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29):25848-58. doi: 10.1074/jbc.M111.253674...
Showing: 161 - 180 of 459 results