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We narrowed to 456 results for: val

Showing: 161 - 180 of 456 results

  2. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...educational materials, protocols, and shared data, on our values of transparency, reproducibility, and community-building... use in brain imaging (Andrews et al., 2019), validated in both western blot and immunohistochemistry....

  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...robust expression of high G+C content genes, as validated through monitoring the expression of fluorescent...sensors New AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More ...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...developed multifunctional fibers, embedded, and validated simultaneously in the brain and the intestines...expression of the opsin in the target neurons, validating the functionality of these inputs and outputs...

  5. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...Myc was developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit... brought about by the tag so it’s important to validate tagged protein functionality (Arribere, et al)...

  6. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... the outcome of the project, or illustrates the value of the work and then build a story around them. ...way at the bottom of my story” and that’s a very valid critique! But do you remember earlier when I said...

  8. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause double-stranded...a natural context, it’s hypothesized that base removal helps prevent the invading genome from “recovering...

  9. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once you’ve used CRISPR/Cas9...Melina Fan guides us through the basics of the validation process for CRISPR mediated genome engineering...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis. While setting up their...retained their stemness, indicating that iTOP may be valuable for CRISPR/Cas9 stem cell editing. The ...

  11. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Control AAV For many AAV experiments, we recommend validating the serotype in your target cells before conducting... wide array of promoters and fluorophores for validating and testing purposes. We will continue to add...

  14. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Appropriate fragment and primer design results in covalently joined, scarless DNA – perfect for transforma...Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids ...

  15. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...research because they provide rapid access to validated reagents, especially during a time when these ... maintain repositories’ capacity for storing, validating, and distributing such reagents are all ways ...

  17. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...track habits, like writing. It’s basically the equivalent of crossing off days on the calendar after completing...and then take a 5-minute break. Each 30-minute interval is called a “Pomodoro.” After four Pomodoros, ...

  18. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...likelihood of ITR deletions occurring.  How do I validate my ITR sequences?  Next-generation sequencing ... the enzymes SmaI and SrfI can also be used to validate ITR sequences as these enzymes cut at known sites...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...engineering Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson...detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington...

  20. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...project. New England Biolabs and Alnylam see the value to the scientific community in accelerating the ...lab from Janelia Research Campus have provided valuable insight into AAV production. Finally, we are ...
Showing: 161 - 180 of 456 results