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We narrowed to 24 results for: Flp recombinase

Showing: 1 - 20 of 24 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named after its ability...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes FRT...post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first..., under the control of Flp recombinase together with inducible Cre recombinase (CreERT2), visualizes individual... al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression...Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels...

  2. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ... using multiple recombinases. To make multiple, precise genome edits, the recombinases used must have ...cross-reactivity, as well as low toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...however, the other recombinase/reporter pairs were inactive, even at very high recombinase levels. These data...toxicity of these recombinases by crossing flies homozygous for a UAS-driven recombinase with flies heterozygous... For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4,... Rubin’s work shows that the yeast recombinases KD, B2, B3 and FLP can be used together in Drosophila ...

  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... EGFP Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory...offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase AAV inventory...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional...inventory here New recombinases Plasmid Serotype Name 55637  AAVrg pAAV-EF1a-Flpo 87306  AAV1 AAV...pAAV-hSyn-EGFP Recombinases Plasmid Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1...detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional studies And more! ...AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. The P1 bacteriophage...components for use in genome manipulation. Cre recombinase, originally named because it “causes recombination...although later referred to as the “cyclization recombinase”), is a 38 kDa protein responsible for intra-...How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event...such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in specified cells or at...in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed... conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) ...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...orientation specificity of the site-specific recombinases (SSRs) Cre and FLP. SSRs bind DNA at target sites to induce...specific recombination events: Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence...pair of FRT sites and include both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen...orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA fragment...absence of, or mCherry in the presence of Cre recombinase.   Once the SSR of choice is introduced, ...events are impossible even in the presence of Cre recombinase. This plasmid now specifically drives expression...about a Revolution: The Impact of Site-Specific Recombinases on Genetic Analyses in Mice. 2004. PubMed PMID...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as Cre-lox allow for more...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and ...expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid...expression. Plasmid 75470 is now available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry...and construct are coexpressed. Three new recombinases from the Cepko Lab are now available as AAV1. CRE-...pAAV-EF1a-C-CreintG Connie Cepko 75469 AAV1 pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon...targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein...

  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors...recognized by Flp), to either flip or excise a gene that is located between them. Early recombinase-dependent...system is off-target or spurious expression of recombinase-dependent AAV vectors. In this article, we will...minimize it. Overview of DIO/FLEx vector design Recombinase-dependent vectors utilize pairs of specific sequences... to prevent transcription in cells where the recombinase was not present (Kuhlman, SK. 2008). While theoretically...spurious expression?   Figure 1.  Cre-recombinase binds to either pair of lox sites, let's say ...This spontaneous recombination persists even in recombinase deficient E. coli and under conditions used to...

  8. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...inventory of recombinases and recombinase-dependent vectors. We recently released several recombinases in various...DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1) Viral...Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV Targeting and manipulating specific, genetically-defined...collection. Of note, we are excited to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See our new ...

  9. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... neurons or you might use it in combination with Flp-FRT. Additionally, you could use the retrograde form... cre-recombinase...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection...only become activated in the presence of Cre recombinase, which is limited to the hippocampus. Thus, only...titers for efficient retrograde transport. For recombinase-dependent vectors, this can cause challenges ...projection pathway neurons and then inject a recombinase-dependent chemogenetic vector directly into the region...

  11. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...-DIO-EGFP Recombinases Plasmid Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA ...pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9...our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options...

  12. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...including our first far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype...New serotype pENN.AAV.CMVs.Pl.Cre.rBG AAV5 Recombinase James M. Wilson New serotype AAVrg = AAV ...

  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ... an alternative to the conventional Cre or Flp recombinases used to excise markers. It has the advantage...

  15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT ...FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to label distinct...-responsive CreERT, the coupling of Cre-lox with FLP-FRT recombination to generate additional recombination...143(1):134-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide...

  17. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Calcium...Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV ...

  18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ... is achieved through site-specific recombinases such as Cre or Flp that induce DNA recombination at defined... "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents." Scientific reports...recombination. Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry 259.3...

  19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction ...marker, likely by breeding to a mouse line with a recombinase. If it turned out your chimeric mice did not ...ssDNA. Here, we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018)...

  20. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    .... We have expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral...
Showing: 1 - 20 of 24 results