We narrowed to 33 results for: Folding Reporter GFP

FlipGFP is a genetically encoded caspase reporter that contains a flipped beta strand of GFP. There is no fluorescence unless the caspase cuts the cleavage site between the strands.

May 21, 2019, 12:10 p.m.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

May 15, 2014, 3:33 p.m.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

April 10, 2018, 12:56 p.m.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Aug. 23, 2018, 12:05 p.m.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

May 26, 2020, 1:15 p.m.

SunTag amplifies fluorescent signals by recruiting multiple copies of GFP. SunTag can help you turn up the brightness in your fluorescent imaging experiments.

March 28, 2017, 2:30 p.m.

Exciting new plasmids, viruses, and antibodies in the Addgene collection!

Jan. 31, 2023, 2:15 p.m.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

Tips on how to choose compatible fluorescent proteins for multi-color imaging.

Oct. 9, 2014, 3 p.m.

mNeonGreen is a bright green fluorescent protein. Along with mWasabi and mTFP, it can be used for traditional imaging, FRET, and multicolor imaging.

April 25, 2019, 3:01 p.m.

Opto-nanobodies (OptoNBs) are nanobodies that can be controlled by light. Learn how you can use OptoNBs to purify proteins or to modulate cell signaling.

Nov. 19, 2019, 2:08 p.m.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Aug. 19, 2014, 3:39 p.m.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Feb. 8, 2018, 12:17 p.m.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

April 11, 2017, 2:30 p.m.

Photoactivatable fluorescent proteins can be switched on (and sometimes off) enabling a variety of techniques and applications.

April 25, 2017, 2:30 p.m.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

Learn how you can use fluorescent biosensors to detect small molecules and other compounds of biological significance in your experiments

May 18, 2017, 2:30 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.