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We narrowed to 35 results for: Tight TRE

Showing: 1 - 20 of 35 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is absolutely necessary...induction system is well-characterized and quite tight (Giacalone et al., 2006). The Chen lab demonstrated...routinely clone the purified amplicons upstream and downstream of the desired deletion directly into cut...transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...accessibility, selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." Biotechniques...

  2. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually...academic calendar Academic researchers must be extreme self-starters and especially good at self-promoting—No...especially with peer mentors, to discuss your strengths, weaknesses, interests and passions is another...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline response element... region from trpB and -10 region from lac. Very tight regulation. Good for modulating gene expression ...are about 100 to 1000 base pairs long and found upstream of their target genes. The sequence of the promoter...of other promoter elements, and tend to be more tightly regulated. Common promoters for eukaryotes and ...induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good for modulating...increases expression of the repressor 10x, thus tightening regulation of lac promoter. Good for modulating...

  4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ....  Viral vector injection Viral vectors allow a tight spatial and temporal control of gene expression ...into the experimental setup, the mouse becomes stressed. It will likely move around faster in the following...some time to adapt to a testing apparatus. General stress levels of the test animal may also be reduced by...noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider:... groups), health status, diet, cage enrichment, stress level, origin (vendor or in-house mouse breeding...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...other BSL-2 materials, you’ll need to use aerosol-tight caps, compatible with your centrifugation speed,...does happen, ensure you can receive appropriate treatment and/or monitoring. Virus-specific concerns   ...

  6. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...and Makerspaces back in 2007, which function as centres for peer learning and knowledge sharing, in the...

  7. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...approach should you use if the budget is particularly tight? Most cloning processes will require a PCR reaction... game, however, or simply looking for a more streamlined approach, you might want to give Gateway a try...cloning kits can often make the process much more streamlined, because they will come with comprehensive and...your favor. For starters, Golden Gate cloning is extremely efficient due to the use of the versatile type...

  9. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...FLSA changes, including finding money in already tight lab budgets and even simply being able to categorize...profoundly shift the academic science landscape: the treatment of trainee scientists as workers, at least for...

  10. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...linked in groups of seven as a Tet Response Element (TRE)), allowing transcription to proceed. When tetracycline...Tet systems function well in mammalian cells, and TRE-containing promoters can be used in the repressible...References and Resources References Gossen M, Bujard H. Tight control of gene expression in mammalian cells by...GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental stage-specific ...uncharacterized promoters. Similarly, placing UAS upstream of a transgene permits directed expression of ...

  11. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ... represents a straightforward means of exerting tight control over Cas9 nuclease activity while eliminating...conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools...complex. When this complex is directed to a target upstream of the transcriptional start site (TSS) of a gene...competent to cut DNA. When directed to a site downstream of the transcriptional start site of a gene of...

  12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...meters long. You twist and roll the string into a tight ball. Now the two ends of the string, instead of...and stop. For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome... Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help of genome...light on dark matter Precise knowledge of which stretches of the genome are pillars for stem cells, or metastasized...

  13. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...times during a 10 minute conversation. Leaving a tight-knit team like that can and will be a little gut-wrenching...question today, because I sometimes wish for a stretch of time that doesn’t involve critical thinking....

  14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...you to easily use a set of plasmids together on a tight budget. To help popularize our kit resources,  ...leader sequences (encoding small leader peptides) downstream of which you can clone your gene of interest....transcription from resulting in the production of a downstream peptide, the transcription terminators in this...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness in cell culture...fluorescence images of C2C12 cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with...Biotech. 2013. Dec 23.   Highly bright and extremely dim GFPs The availability of the three dimensional...unprecedentedly-high brightness, bfloGFPa1, and the other with extremely-dim brightness, bfloGFPc1. The plasmids used for...and Keith Shearwin labs have developed a new, streamlined approach to genetic engineering which drastically...plasmid after transformation. Addgene depositors Sylvestre Marillonnet and Nicola Patron have assembled two... N- and C-terminal tags (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for ...

  16. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... (TMP-tag) (Miller et al., 2005). Although this tight interaction is not covalent, later work showed that...cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility comes at ..., Article 4. https://doi.org/10.1038/nmeth749 Strebinger, D., Frangieh, C. J., Friedrich, M. J., Faure...

  17. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...the contents. Tightly coupling barcodes and containers might not be such a bad idea, tightly coupling them...could be treating these pieces of data in ways that are inconsistent with how they are being treated in the...think carefully about how you want your software to treat those encoded numbers. Loosely couple containers...In the first example we’ve used inheritance to tightly couple the contents of a tube with the tube itself...

  18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...drawbacks while considering this treatment as the duration of treatment can affect the cell growth and ... and antibacterial drugs like penicillin and streptomycin. Mycoplasma can pass through filtration methods...media as much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid...exposure of the cell culture to air and make sure to tighten the flask cap before placing them in the incubators...agents can be used to wash the cell cultures. The treatment can take a few weeks to a couple of months depending...

  19. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus pyogenes.   Figure 2:  Crystal structure...purple; sgRNA, dark blue.   The REC lobe binds tightly to the repeat:anti-repeat duplex of the guide RNA... It’s not actually clear! In vitro, Cas9 holds tightly to DNA even after cleaving it, so many researchers...

  20. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein... interest. Once the complex is captured, it is treated so that the protein and DNA are no longer bound... be isolated and studied through a variety of downstream methods, such as qPCR or next-gen sequencing.... the upsteam sample collection process or the downstream analysis process. And ChIP itself begins at.....between 200-1000 bp. This is to ensure that your downstream analysis focuses on the DNA your protein is actively...protein:DNA complexes. Your DNA is now ready for downstream analysis via any methods suitable for isolated...attach the proteins to the DNA, depending on how tightly the proteins bind natively. But the amount of cross-linking...
Showing: 1 - 20 of 35 results